Articles in PresS. Am J Physiol Renal Physiol (November 4, 2015). doi:10.1152/ajprenal.00345.2015
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RENAL DENERVATION ATTENUATES NADPH OXIDASE-MEDIATED
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OXIDATIVE STRESS AND HYPERTENSION IN RATS WITH HYDRONEPHROSIS
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Maria Peleli1*, Ammar Al-Mashhadi2,3*, Ting Yang1, Erik Larsson4, Nils Wåhlin5, Boye L.
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Jensen6, A. Erik G. Persson3, Mattias Carlström1
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Dept of Physiology & Pharmacology, Karolinska Institutet, Stockholm, Sweden
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Dept of Women's and Children's Health, Div. of Pediatric Surgery, Uppsala University,
Sweden 3
Dept Medical Cell Biology, Uppsala University, Uppsala, Sweden
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Dept of Genetics and Pathology, Uppsala University, Uppsala, Sweden
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Department of Pediatric surgery, Astrid Lindgren hospital, Karolinska Institutet, Stockholm,
Sweden 6
Dept of Physiology & Pharmacology, University of Southern Denmark, Odense, Denmark
* Shared 1st authors
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Short title: Denervation attenuates oxidative stress & hypertension
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Word count (Full manuscript excl. Tables & Figure Legends): 6927
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Word count (Abstract): 250
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Tables: 2
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Figures: 7
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Correspondence:
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Mattias Carlström
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Dept. of Physiology and Pharmacology, Nanna Svartz Väg 2, S-17177, Stockholm, Sweden
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Phone: +46-(0)8-52487924
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Fax: +46-(0)8-332278
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E-mail:
[email protected]
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1 Copyright © 2015 by the American Physiological Society.
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ABSTRACT
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Aim: Hydronephrosis is associated with development of salt-sensitive hypertension. Studies
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suggest that increased sympathetic nerve activity (SNA) and oxidative stress play important
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roles in hypertension and modulation of salt-sensitivity. This study primarily aimed to
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examine the role of renal SNA in the development of hypertension in rats with
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hydronephrosis. In addition, we aimed to investigate if NADPH oxidase (NOX) function
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could be affected by renal denervation.
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Methods: Partial unilateral ureteral obstruction (PUUO) was created in 3-weeks old rats to
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induce hydronephrosis. Sham surgery or renal denervation was performed at the same time.
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Blood pressure was measured during normal, high and low salt diets. Renal excretion pattern,
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NOX activity and expression, as well as components of renin-angiotensin-aldosterone-system
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(RAAS) were characterized after treatment with normal salt diet.
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Results: On normal salt diet, PUUO rats had elevated blood pressure compared with controls
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(115±3 vs 87±1 mmHg, p
