Asian Pacific Journal of Cancer Prevention, Vol 15, 2014. 717 ... and tobacco habits with reference to risk of stomach cancer in Mizoram, India. A total of 105 ...
DOI:http://dx.doi.org/10.7314/APJCP.2014.15.2.717 p53 Codon 72 Polymorphism Interactions with Dietary and Tobacco Related Habits and Stomach Cancer Risk in Mizoram
RESEARCH ARTICLE p53 Codon 72 Polymorphism Interactions with Dietary and Tobacco Related Habits and Risk of Stomach Cancer in Mizoram, India Mridul Malakar1,3, K Rekha Devi1, Rup Kumar Phukan1, Tanvir Kaur2, Manab Deka3, Lalhriat Puia4, Lalrinliana Sailo4, T Lalhmangaihi4, Debajit Barua5, Sanjib Kumar Rajguru1, Jagadish Mahanta1, Kanwar Narain1* Abstract Background: This study was carried out to investigate the interaction of p53 codon 72 polymorphism, dietary and tobacco habits with reference to risk of stomach cancer in Mizoram, India. A total of 105 histologically confirmed stomach cancer cases and 210 age, sex and ethnicity matched healthy population controls were included in this study. Materials and Methods: The p53 codon 72 polymorphism was detected by PCR-RFLP and sequencing. H. pylori infection status was determined by ELISA. Information on various dietary and tobacco related habits was recorded with a standard questionnaire. Results: This study revealed that overall, the Pro/ Pro genotype was significantly associated with a higher risk of stomach cancer (OR, 2.54; 95%CI, 1.01-6.40) as compared to the Arg/Arg genotype. In gender stratified analysis, the Pro/Pro genotype showed higher risk (OR, 7.50; 95%CI, 1.20-47.0) than the Arg/Arg genotype among females. Similarly, the Pro/Pro genotype demonstrated higher risk of stomach cancer (OR, 6.30; 95%CI, 1.41-28.2) among older people (>60 years). However, no such associations were observed in males and in individuals 60 (total=156 nos.)
Controls, n (%)
Male Female Total ≤60 >60 Arg/Arg no. (%) 11 (10.5) 36 (17.1)
Total
Cases, n (%)
9 (11.1) 2 (8.3) 8 (14.0) 3 (6.2)
Control 27 (16.7) 9 (18.8) 19 (18.6) 17 (15.7)
Case
Control
Case
44 (54.3) 12 (50.0) 31 (54.4) 25 (52.1)
77 (47.5) 33 (68.8) 50 (49.0) 60 (55.6)
28 (34.6) 10 (41.7) 18 (31.6) 20 (41.7)
Control 58 (35.8) 6 (12.5) 33 (32.4) 31 (28.7)
Table 2. p53 Codon 72 Genotypes, Tobacco & Different Dietary Habits and Risk of Stomach Cancer in Mizoram, India Characteristics Categories Cases, n (%) Controls, n (%) Univariate OR* p-value Multivariate Adjusted (95 % CI) OR (95 % CI) p53 Genotype Arg/Arg Arg/Pro Pro/Pro Tobacco Smoking Non-Smoker Smoker Tuibur habit Non-User User Smoke dried fish taking habit No Yes Preserved meat (smoked/sun dried) No consuming habit Yes Fermented pork fat (sa-um)# No taking habit Yes Preserved bamboo shoot taking habit No Yes Fermented soya-bean (bekang) No ¥ taking habit Yes
11 (10.5) 56 (53.3) 38 (36.2) 21 (20.0) 84 (80.0) 81 (77.1) 24 (22.9) 8 (7.6) 97 (92.4) 5 (5.7) 99 (94.3) 8 (7.6) 97 (92.4) 7 (6.9) 98 (93.3) 10 (9.5) 95 (90.5)
36 (17.1) 110 (52.4) 64 (30.5) 85 (40.5) 125 (59.5) 177 (84.3) 33 (15.7) 67 (31.9) 143 (68.1) 44 (21.0) 166 (79.0) 35 (16.7) 175 (83.3) 26 (12.4) 184 (87.6) 39 (18.6) 171 (81.4)
1.0 (ref) 1.71 (0.81-3.63) 0.16 2.04 (0.90-4.62) 0.08 1.0 (ref) 2.90 (1.63-5.17)
