Sep 22, 2006 - Department of Marine Biology, College of Marine Life Sciences, Ocean University of China, Qingdao .... For resuscitation of the VBNC cells in animal .... Science Foundation of China (30371108) and National High-Tech R & .... Edwardsiella tarda from aquatic animal species and surface waters in Florida.
Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate䌤 Meng Du,1 Jixiang Chen,1* Xiaohua Zhang,1 Aijuan Li,1 Yun Li,1 and Yingeng Wang2 Department of Marine Biology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, People’s Republic of China,1 and Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, People’s Republic of China2 Received 22 September 2006/Accepted 12 December 2006
Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant throughout the 28-day period by acridine orange direct counting, while plate counts declined to undetectable levels (
