AJP-Renal Articles in PresS. Published on August 21, 2001 as DOI 10.1152/ajprenal.0064.2001
1 Role of Cytochrome P450 Arachidonate Metabolites in Endothelin Signaling in the Rat Proximal Tubule
Bruno A. Escalante,1 John C. McGiff2 and Adebayo O. Oyekan3
1
Centro de Investigacion y de Estudios Avanzados IPN, Av
Institutio Politecnico Nacional 2508 Mexico, D.F. 07300; 2Department of Pharmacology, New York Medical College, Valhalla, New York 10595; and 3College of Pharmacy and Health Sciences, Texas Southern University, Houston, Texas 77004
Running Head: Proximal tubular endothelin-eicosanoid interactions
Address correspondence to: John C. McGiff, M.D. Professor and Chairman Department of Pharmacology New York Medical College Valhalla, NY 10595 Phone: 914-594-4075 Fax: 914-347-4956 e-mail:
[email protected]
Copyright 2001 by the American Physiological Society.
2 Abstract We examined the rat proximal tubule (PT) response to endothelin-1 (ET-1) in terms of 20-HETE dependency. Arachidonic acid (AA) (1 µM) decreased ouabainsensitive
Rb uptake from 2.1+0.1 to 0.3+0.08 ng Rb/10 µg
86
protein/2 min (p
