RS RS RS - PLOS

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J-ia. 3-1f D-3r2 D-3r M-2b. (DB1A)SJ tags1. tagM. tagM. (DB1A) ESJ. S-3a S-4a. M-2b. M-1b. Dter. (KI/JK3)SJ. (KI/JK3) ESJ. P-ia K-in l-in J-ex. (KIA9/JK3)SJ.
(Jδ1/Dδ3) ESJ

tagS1

(Jδ1/Dδ3)SJ J-ia

J-1f

tagS1

(Dβ1∆) ESJ

D-3r2 D-3r M-2b

(Dβ1∆)SJ

S-3a S-4a

M-2b

M-1b

(KI/Jκ3)SJ P-ia K-in

J-in J-ex

Donor substrates (ESJ, 23-RSS)

(KI∆9/Jκ3)SJ

(KI∆9/Jκ3) ESJ

P-ea P-ia

J-in J-ex

(KI/Jκ3∆9)SJ

(KI/Jκ3∆9) ESJ

P-ia K-in

tagM M-1b

M-1b

P-ib P-eb

Dδ3 M-2b

tagS2

D-3f

tagM

Dβ1

tagM

Dter

(KI/Jκ3) ESJ

Dδ3

tagM

D-3r S-8a S-7a

Dβ1 M-2b

tagS2

Dter

S-8a S-7a

tagS1 (Dβ1/Dd1)SJ

(Dβ1/Dδ1)12 ESJ

S-3a S-4a

tagM

M-2b

M-1b

tagS1 (Dδ1/Dβ1)SJ

(Dβ1/Dδ1)23 ESJ

S-3a S-4a

tagM

M-2b

M-1b

Jβ2.7

Jβ2.7

J-5a J-6a

Hprt

Hprt + VκA27

VκA27 tagC

H-7a H-8a

Hprt Hprt + Jβ2.7 Hprt + LMO2

Y-1a X-1a

Jβ2.7

H-7a H-8a

Hprt

LMO2

Hprt

L-5b

TAL2 tagC

H-7a H-8a

200 bp

tagC C-3b

H-7a H-8a

Hprt + TAL2

Target substrates (12-RSS)

S-rn C-3b

C-3b

M

Sc/N

RS

tagC