Supplementary Figure 6 - Cell composition in naive spleens of WT and CatB-/- mice. Splenocytes from naive WT and Cat-/- mice were stained for CD4, CD25 ...
May 4, 2015 - ASAâAmerican Society of Anesthesiology; CEAâCarotid endarterectomy; RINDâReversible ..... Creager, M.A.; Fowler, S.B.; Friday, G.; et al.
Jan 10, 2017 - http://www.aai.org/ji/copyright.html ... Receive free email-alerts when new articles cite this article. ... implying that CD4 T cells in part controlled the differentiation of the CD8 T cell clones responsive to these epitopes during.
Correspondence and reprint requests: Kenneth F. Bradstock, Blood and Marrow Transplant Service, Westmead .... EDTA-PBS and incubated for 15 minutes at 37°C. Cells ..... Orme I, Roberts A, Griffin J, Abrams J. Cytokine secretion by.
Jan 24, 2013 - Erin E. Hillhouse1,2, Jean-Sébastien Delisle2,3,4 and Sylvie Lesage1,2*. 1 Department of .... (Joly and Hudrisier, 2003). Notably, the in vitro ...
Immune Modulation by Zoledronic Acid in Human Myeloma: ... of PBLs but can be expanded by zoledronic acid (ZA)-treated monocytes or dendritic cells (DC).
Dec 25, 2011 - ... Sezione di Farmacologia, Tossicologia e Chemioterapia, Universit`a di Perugia, .... antigen-specific CD8+ T cells after ocular HSV infection.
Jun 4, 2007 - Antibody treatment. Mice were intravenously injected with 1 â 2 mg anti â. IL-6R mAb (MR16-1; a gift from Chugai Pharmaceutical Company ...
involved appears to be the so-called CD4* Th2 subset which produces interleukin-4 (IL-4) and interIeukin-5. (IL-5). In vitro and in vivo experiments have ...
1 Division of Mycobacterial Research, National Institute for Medical ... We studied the contribution of CD4 T cell help to survival and competitive fitness of CD8.
ages of cells were compared between groups. RESULTS: While expression of CCR4 (Th2 marker) on memory CD4+. T cells was high in both groups (>35%), ...
Jul 29, 2014 - Soto-Nieves N, Puga I, Abe BT, Bandyopadhyay S, Baine I, Rao A, ... Baine I, Abe BT, Macian F (2009) Regulation of T-cell tolerance by.
Feb 2, 2017 - EDTA (Titriplex III) (Merk KG, Darmstadt, Germany) ...... Bruno L, Fehling HJ, and von Boehmer H: The alpha beta T cell receptor can replace.
Sci. USA. Vol. 89, pp. 5557-5561, June 1992. Immunology. CD4 and CD8 regulate interleukin 2 responses of T cells. (Lyt-2/L3T4/p56I&/protein-tyrosine kinase).
JOHN J. DONNELLY, AND MICHAEL J. CAULFIELD*. Department of Virus and Cell Biology, Merck Research Laboratories, West Point, Pennsylvania 19486.
chased from Charles River Laboratories (Raleigh, N.C.). Animals were ..... 2:1847â1851. 5. Deck, R. R., C. M. DeWitt, J. J. Donnelly, M. A. Liu, and J. B. Ulmer.
Jul 19, 2010 - CpG 1826 was obtained from CyberGene (Stockholm, Sweden). Cell lines. D2F2/E2 cells (H-2d) expressing the human Her2/neu protein.
Aug 29, 2016 - Derek DC Ireland, Cecilia Tami, Joao Pedras-Vasconcelos and Daniela Verthelyi. Neonates are at increased risk of viral encephalopathies that ...
ZHOU XING,* JUN WANG, KENNETH CROITORU, AND JULIA WAKEHAM ..... Histopathology of lungs from CD4 / (a), CD8 / (b), C57BL/6 (c), IL-12 p40 / (d), and SCID .... Cooper, A. M., D. K. Dalton, T. A. Stewart, J. P. Griffin, D. G. Russell, and.
bination of anti-CD58 and anti-CD2 mAb (5. µg/mL). (D). Results are expressed as pg/mL of the indicated cytokine evaluated with a fluores- cence cytokine kit ...
cisco, USA). 2.3. ... breast tissue (CD4+ cells: 1130 ± 122 cel/mm2 and CD8+ ... 6000. 8000. Stromal CD4+ and CD8+ T cells density. Normal breast. 1130. 457.
Aug 6, 2001 - Mischo Kursar,1 Kerstin Bonhagen,2 Joachim Fensterle,1 Anne Köhler,1. Robert Hurwitz,1 Thomas Kamradt,2 Stefan H.E. Kaufmann,1.
Apr 15, 2016 - French, J.J., Cresswell, J., Wong, W.K., Seymour, K., Charnley, R.M., and Kirby, J.A. (2002). .... and Greenberg, P.D. (2002). Adoptive T cell ...
CD27-), and terminally differentiated effector memory (TEMRA; CD45RA+CCR7-CD27-) within the total. CD4 or CD8 T cell population. C. T cell activation was ...
S1 Fig. Single cell (gate)
CD4+ T cells
CD4
FSC-A
Lymphocytes (gate)
SSC-A
A
FSC-H
CD3
CD8+ T cells
CD8
FSC-A
CD3
B CD4+ T cells
CD8+ T cells N
CD27
CD45RA
CD27
CD45RA
N
TEMRA
CCR7
TEMRA
CCR7
CCR7 TM
CM
CD27
CM
CD27
TM
CCR7
EM
EM
CCR7
CCR7
CD4+ T cells %PD1+ (gate)
CD38
CD27
PD1
CD27
%CD57+ (Q1+Q2) %CD57+ of CD28- (Q1/(Q1+Q4)
CD57 CD28
%PD1+ (gate)
CD38
%CD27-/CD28- (Q8)
CD27
CD57
%CD57+ (Q1+Q2) %CD57+ of CD28- (Q1/(Q1+Q4))
CD28
%HLA-DR+/CD38+ (Q2)
%CD27-/CD28- (Q8)
CD27
PD1
HLA-DR
%HLA-DR+/CD38+ (Q2)
CD8+ T cells
HLA-DR
C
CD28
CD28
S1 Fig. Gating strategy flow cytometry. A. Gating of CD4+ and CD8+ T cells. B. T cell differentiation was defined as the proportion of naïve (N; CD45RA+CD27+CCR7+), central memory (CM; CD45RACCR7+CD27+), transitional memory (TM; CD45RA-CCR7-CD27+), effector memory (EM; CD45RA-CCR7CD27-), and terminally differentiated effector memory (TEMRA; CD45RA+CCR7-CD27-) within the total CD4 or CD8 T cell population. C. T cell activation was defined as the proportion of cells that were positive for both CD38 and HLA-DR within the total CD4 or CD8 T cell population. T cell exhaustion was defined as the proportion of PD1 positive cells within the total CD4 or CD8 T cell population. Terminally differentiated T cells were defined as proportion of CD57 positive cells within the total CD4 or CD8 T cells population, the proportion of cells negative for both CD27 and CD28 within the total CD4 or CD8 T-cells population, or the proportion of CD57 positive within the CD28-CD4+ or CD28-CD8+ T cell population.
