S2 Table: Genotype, virulence genes, phage patterns

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Amp. R. SH044-1. 2005 t127. 07-23-21-16-34-33-13. ND. CC1. a h k q ...... protein; scn, staphylococcal complement inhibitor gene; AmpR, ampicillin resistance.
S2 Table: Genotype, virulence genes, phage patterns and ampicillin resistance of colonizing S. aureus isolates from healthy blood donors.

1

Sa1int

Sa2int

Sa3int

Sa4int

Sa5int

Sa6int

Sa7int

sea

sep

sak

chp

scn

AmpR

egc SAgs gimno gimno gimno gimno gimno gimno gimno gimno gimno gimno gimno gimno gimno gimno gimno gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimnou gimno gimno gimno gimno gimno gimno gimno gimno gimno gimno gimno imnou gimnou gimnou gimno -

mecA

MLST ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ST45 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ST395 ST395 ND ND ND ND

pvl

spa repeats 07-23-21-16-34-33-13 07-23-21-17-13-34-34-16-34-33-13 26-23-17-34-17-20-17-12-17-16 26-23-17-34-17-20-17-12-17-16 26-23-17-34-17-20-17-12-17-16 26-23-17-34-17-20-17-12-17-16 26-23-17-34-17-20-17-12-17-16 26-23-17-34-17-20-17-12-17-16 26-23-17-34-17-20-17-12-17-16 07-23-21-17-34-12-23-02-12-23 07-23-21-17-34-12-23-02-12-23 07-23-21-17-34-12-23-02-12-23 07-23-21-17-34-12-23-02-12-23 11-19-12-21-17-34-24-34-22-25 11-19-12-21-17-34-24-34-22-25 11-19-12-21-17-34-24-34-22-25 11-19-12-21-17-34-24-34-22-25 11-19-12-21-17-34-24-34-22-25 11-19-12-21-17-34-24-34-22-25 11-19-12-21-17-34-24-34-22-25 04-21-17-34-24-34-22-25 04-21-17-34-24-34-22-25 04-21-17-34-24-34-22-25 11-19-12-34-22-25 07-23-12-33-22-17 07-23-12-33-22-17 07-23-12-34-34-12-12-23-02-12-23 07-23-12-34-34-12-12-23-02-12-23 07-23-12-34-34-12-12-23-02-12-23 07-23-12-34-34-12-12-23-02-12-23 07-23-12-34-34-12-12-23-02-12-23 07-23-12-34-34-12-12-23-02-12-23 07-23-12-34-34-12-23-02-12-23 07-23-12-12-34-34-12-12-23-02-12-23 07-23-12-34-12-12-23-02-12-23 26-23-13-23-31-05-17-25-17-25-16-28 26-23-13-23-31-05-17-25-17-25-16-28 26-23-29-17-25-17-25-28 26-17-25-17-25-16-28 04-21-12-41-20-17-12-12-17 04-21-12-41-20-17-12-12-17 04-21-12-41-20-17-12-12-17 04-21-12-41-20-17-12-12-12-17 15-12-16-02-16-02-25-17-24-24 15-12-16-02-16-02-25-17-24-24 15-12-16-02-16-02-25-17-24-24 15-12-16-02-16-02-25-17-24-24 15-12-16-02-16-02-25-17-24-24 15-12-16-02-16-02-25-17-24-24 15-12-16-02-16-02-25-17-24-24 15-12-16-02-16-02-25-17-24-24 15-12-16-02-16-02-25-17-24-24 15-12-16-02-16-02-25-17-24-24 15-12-16-16-02-16-02-25-17-24-24 15-12-16-02-16-02-25-17-24-24-24 08-16-02-16-02-25-17-24 15-12-16-02-16-02-25-17-24 08-16-02-16-02-25-17-24-24 08-16-02-16-02-25-17-24-24 08-16-02-16-02-25-17-24-24 15-12-16-16-02-25-17-24 15-12-16-16-02-25-17-24 15-12-16-02-16-02-25-16-17-24 15-16-02-25-17-24-24 04-44-54-31-12-16-34-16-12-33-34 15-12-16-16-02-16-02-25-17-25 15-12-16-16-16-16-16-02-16-02-25-17-24 15-12-16-02-16-16-02-16-02-25-17-24-24 15-12-16-02-25-17-24-24 15-12-16-16-02-16-02-25-17-24 15-12-16-02-16-02-25-17 15-12-02-16-02-25-17-24-24 08-16-16-02-16-02-25-17-24 04-33-31-12-16-16-12-33-34 04-44-33-31-12-16-34-12-33-34 09-02-16-13-13-17-34-16-34 08-16-02-16-34-13-17-34-16-34 08-16-02-16-34-13-17-34-16-34 08-16-02-16-34-13-17-34-16-34 08-16-02-16-34-34-17-34-16-34 09-02-16-34-13-17-34-16-34 08-104-02-16-34-13-17-34-16-34 08-13-16-34-16-34 08-16-02-83-34-13-17-34-83-34 08-16-02-16-34-13-17-13-16-34 08-16-02-16-34-13-17-13-16-34 04-17-23-24-20-17-25 04-20-12-17-20-17-12-17-17 04-20-12-17-20-17-12-17-17 04-20-12-17-20-17-12-17-17 04-20-12-17-20-17-12-17-17 04-20-12-17-20-17-12-17-17 04-20-12-17-20-17-12-17-17 04-20-12-17-20-17-12-17-17 04-20-12-17-20-17-12-17-17 04-20-12-17-20-17-12-17-17 04-20-12-17-20-17-12-17-17 04-34-17-66-32-17-23-24 08-12-16-34-02-43-34-16-02-17-16 08-12-16-02-43-34-16-16-02-17-16 08-16-02-43-34-16-02-17-16 08-16-02-43-34-16-16-02-17-16 08-16-34-02-43-13-16-16-02-17-16 15-12-16 08-16-34 11-25 121-17-17-17-17

Phage genes

etd

Year 2005 2005 2005 2005 2005 2005 2005 2002 2002 2005 2002 2002 2002 2005 2005 2005 2005 2005 2002 2002 2002 2002 2002 2005 2005 2002 2005 2005 2005 2005 2002 2002 2005 2005 2002 2005 2005 2005 2005 2002 2002 2002 2002 2005 2005 2005 2002 2002 2002 2002 2002 2002 2002 2002 2005 2005 2005 2005 2005 2002 2005 2005 2005 2002 2005 2005 2002 2002 2005 2002 2002 2002 2005 2002 2002 2002 2005 2002 2002 2002 2002 2005 2005 2002 2005 2002 2005 2005 2005 2005 2005 2005 2002 2002 2002 2002 2002 2002 2002 2002 2002 2002 2002 2005 2005 2002 2002

MGE-encoded SAgs ahkq h bkqdjr p p p p p p p djr djr djr cl djr djr djr bkq bkqdjr bqdjr adjr clp clp b b tst a tst a tst a tst tst a tst tst a tst a tst a tst a tst tst a tst a tst tst a h tst a tst tst tst a h tst b h l tst cl acl cl cl cl cl cl cl h a c l tst a c l tst a k q c l tst kq a a cl djr b

eta

Strain ID SH044-1 SH097-2 SH033-1 SH042-1 SH076-1 SH093-1 SH137-2 T009-2 T161-1 SH081-2 T002-2 T009-1 T157-1 SH010-1 SH016-1 SH051-2 SH100-1 SH115-2 T041-1 T051-2 T145-1 T169-1 T198-1 SH131-1 SH059-1 T194-1 SH027-1 SH028-1 SH070-1 SH130-1 T093-1 T099-1 SH126-2 SH019-2 T202-2 SH072-1 SH073-1 SH055-1 SH024-2 T162-1 T171-1 T192-1 T097-1 SH039-1 SH077-1 SH112-2 T043-1 T098-1 T098-2 T100-1 T124-1 T141-1 T190-2 T135-1 SH105-1 SH032-1 SH109-1 SH067-1 SH079-1 T039-2 SH064-1 SH065-2 SH069-1 T061-1 SH020-1 SH106-2 T172-1 T077-1 SH046-1 T169-2 T035-1 T037-1 SH121-1 T200-1 T020-1 T184-2 SH001-2 T052-1 T166-2 T108-1 T085-1 SH024-1 SH056-1 T088-2 SH013-2 T179-1 SH018-1 SH015-1 SH021-2 SH022-1 SH133-1 SH145-1 T011-1 T029-2 T055-1 T105-2 T201-1 T142-2 T148-2 T191-1 T110-1 T166-1 T132-1 SH017-2 SH048-1 T118-1 T054-1

Virulence genes deduced MLST CC1 CC1 CC1 CC5 CC5 CC5 CC5 CC5 CC5 CC5 CC7 CC7 CC7 CC7 CC8 CC8 CC8 CC8 CC8 CC8 CC8 CC8 CC8 CC8 CC8 CC12 CC12 CC15 CC15 CC15 CC15 CC15 CC15 CC15 CC15 CC15 CC22 CC22 CC22 CC22 CC25 CC25 CC25 CC25 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC30 CC34 CC34 CC45 CC45 CC45 CC45 CC45 CC45 CC45 CC45 CC45 CC45 CC45 CC50 CC101 CC101 CC101 CC101 CC101 CC101 CC101 CC101 CC101 CC101 ST182 CC395 CC395 CC395 CC395 CC395 excluded excluded excluded singleton

agr

spa genotyping spa type t127 t1491 t002 t002 t002 t002 t002 t002 t002 t091 t091 t091 t091 t008 t008 t008 t008 t008 t008 t008 t711 t711 t711 t723 t156 t156 t084 t084 t084 t084 t084 t084 t085 t120 t346 t005 t005 t1648 t541 t078 t078 t078 t258 t012 t012 t012 t012 t012 t012 t012 t012 t012 t012 t017 t018 t019 t021 t122 t122 t122 t1504 t1504 t1641 t1642 t1643 t1650 t1660 t1672 t275 t318 t342 t840 t964 t1664 t1670 t004 t015 t015 t015 t050 t065 t1238 t1647 t1666 t302 t302 t246 t056 t056 t056 t056 t056 t056 t056 t056 t056 t056 t493 t1362 t1645 t1651 t1655 t1669 t1209 t026 t118 t1680

3 3 2 2 2 2 2 2 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 1 1 1 1 1 1 1 1 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 1 1 1 1 1 1 1 1 1 1 1 4 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 1 4

-

-

-

+ -

+ + + + + + + + + -

+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + -

+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + -

-

+ + + + + + + + + + + + + + + + + + + + + -

+ + + + + + + -

-

+ + + + + + + + + + + + + + + + + + + + + -

+ + + + + + + + + -

+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + -

+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + -

+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + -

+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + -

spa types were clustered by BURP analysis into CCs and corresponding MLST CCs were deduced using the Ridom database. Key: col, nasal colonization (nasopharyngeal sample); SSTI, skin and soft tissue infection; CRSwNP, chronic rhinosinusitis with nasal polyps; agr, accessory gene regulator; Staphylococcal enterotoxins (SEs) are indicated by single letters (a = sea , etc.). Tst, toxic shock syndrome toxin 1 gene; egc, superantigen genes of the enterotoxin gene cluster, i.e. seg, sei, sem, sen, seo, and seu; eta/etd, exfoliative toxins a and d; luk-PV, Panton-Valentine leukocidine gene; Sa1int, S. aureus integrase type 1; sak, Staphylokinase gene; chp , gene encoding the chemotaxis inhibitory protein; scn, staphylococcal complement inhibitor gene; AmpR, ampicillin resistance