S2 Table - PLOS

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Figure. Relevant genotype a. Strain number. 1A. ESP1. ADR21. ESP1-myc13. ADR2146. pGAL-SWE1 ESP1-13myc. ADR4854. 1C. ESP1. ADR4006 esp1-2A.
S2 Table - Strains used in each figure Figure 1A

1C

1D 1E

1F 2A

2B

2C

Relevant genotype a ESP1 ESP1-myc13 pGAL-SWE1 ESP1-13myc ESP1 esp1-2A esp1-3A esp1-1A esp1-3A+2A esp1-2A+1A esp1-3A+1A esp1-2A+3A+1A cdc55∆ rts1∆ ESP1 esp1-3A ESP1 esp1-2A esp1-3A esp1-1A esp1-3A+2A esp1-2A+1A esp1-3A+1A esp1-2A+3A+1A ESP1 ESP1 esp1-3A PDS1-AID esp1-3A PDS1-AID ESP1-3D PDS1-AID ESP1/ESP1 ESP1 PDS1-AID/ESP1 PDS1-AID ESP1-3D PDS1-AID/ESP1 PDS1-AID ESP1-3D PDS1-AID/ESP1-3D PDS1-AID esp1-3A PDS1-AID/ESP1 PDS1-AID esp1-3A PDS1-AID/esp1-3A PDS1-AID esp1∆ [CEN-ESP1-HIS3] ESP1 [CEN-HIS3] ESP1-3D [CEN-ESP1-HIS3] PDS1-AID [CEN-HIS3]

Strain number ADR21 ADR2146 ADR4854 ADR4006 ADR6716 ADR6715 ADR6714 ADR6717 ADR6718 ADR6774 ADR6775 ADR6876 ADR4215 ADR4006 ADR6715 ADR4006 ADR6716 ADR6715 ADR6714 ADR6717 ADR6718 ADR6774 ADR6775 ADR4006 ADR4006 ADR6715 ADR6387 ADR6956 ADR6448 ADR9600 ADR9606 ADR9608 ADR9602 ADR9610 ADR9604 ADR9408 ADR22 ADR22 ADR6428

2D 3A

3B 3C

4A

4B 5A

PDS1-AID [CEN-ESP1-HIS3] ESP1-3D [CEN-HIS3] ESP1-3D [CEN-ESP1-HIS3] ESP1-3D PDS1-AID [CEN-HIS3] ESP1-3D PDS1-AID [CEN-ESP1-HIS3] esp1-3A [CEN-HIS3] esp1-3A [CEN-ESP1-HIS3] esp1-3A PDS1-AID [CEN-HIS3] esp1-3A PDS1-AID [CEN-ESP1-HIS3] PDS1-AID SPC42-eGFP ESP1-3D PDS1-AID SPC42-eGFP wild-type PDS1-AID cdc55∆ swe1∆ cdc55∆ PDS1-AID swe1∆ PDS1-AID swe1∆ cdc55∆ swe1∆ cdc55∆ PDS1-AID cdc55∆ PDS1-AID SPC42-eGFP swe1∆ cdc55∆ PDS1-AID SPC42-eGFP PDS1-AID ura3::240lacO pCUP1-eGFP-lacI SPC42-mCherry cdc55∆ PDS1-AID ura3::240lacO pCUP1eGFP-lacI SPC42-mCherry ESP1-3D PDS1-AID ura3::240lacO pCUP1eGFP-lacI SPC42-mCherry wild-type cdc55∆ cdc55∆ swe1∆ mcd1-10A mcd1-10A cdc55∆ PDS1-AID cdc55∆ PDS1-AID mcd1-10A PDS1-AID cdc55∆ esp1-3A PDS1-AID cdc55∆ swe1∆ esp1-3A PDS1-AID cdc55∆ mcd1-10A PDS1-AID cdc55∆ esp1-3A mcd1-10A PDS1-AID cdc55∆ swe1∆ esp1-3A mcd1-10A PDS1-AID swe1∆ cdc55∆ PDS1-AID CDC14-eGFP SPC42-mCherry wild-type

ADR6428 ADR6409 ADR6409 ADR6450 ADR6450 ADR7195 ADR7195 ADR6863 ADR6863 ADR6389 ADR6446 ADR22 ADR6428 ADR6436 ADR7138 ADR6426 ADR7143 ADR7140 ADR7145 ADR6325 ADR7043 ADR6724 ADR6722 ADR6886 ADR22 ADR6436 ADR7140 ADR6635 ADR6639 ADR6428 ADR6426 ADR6636 ADR6870 ADR7149 ADR6642 ADR9406 ADR9404 ADR7205 ADR6431

5B

5C 5D

6A

6B 7

8

slk19∆ ESP1-3D PDS1-AID slk19∆ ESP1-3D slk19∆ PDS1-AID ESP1-3D PDS1-AID slk19∆ ESP1-3D PDS1-AID wild-type slk19∆ PDS1-AID cdc55∆ slk19∆ PDS1-AID slk19∆ cdc55∆ cdc55∆ PDS1-AID slk19∆ cdc55∆ PDS1-AID slk19∆ PDS1-AID SPC42-eGFP pCUP1-eGFP-lacI ura3::240lacO SPC42mCherry ESP1-3D pCUP1-eGFP-lacI ura3::240lacO SPC42-mCherry cdc55∆ pCUP1-eGFP-lacI ura3::240lacO SPC42-mCherry PDS1-AID pCUP1-eGFP-lacI ura3::240lacO SPC42-mCherry ESP1-3D PDS1-AID pCUP1-eGFP-lacI ura3::240lacO SPC42-mCherry cdc55∆ PDS1-AID pCUP1-eGFP-lacI ura3::240lacO SPC42-mCherry slk19∆ PDS1-AID pCUP1-eGFP-lacI ura3::240lacO SPC42-mCherry wild-type PDS1-AID cdc55∆ swe1∆ PDS1-AID ESP1-3D PDS1-AID PDS1-AID slk19∆ PDS1-AID PDS1-AID SMC3-GFP SPC29-RFP cdc55∆ PDS1-AID SMC3-GFP SPC29-RFP cdc55∆ swe1∆ PDS1-AID SMC3-GFP SPC29RFP ESP1-3D PDS1-AID SMC3-GFP SPC29-RFP slk19∆ PDS1-AID SMC3-GFP SPC29-RFP PDS1-AID

ADR8230 ADR8229 ADR6428 ADR8231 ADR8233 ADR6450 ADR8232 ADR22 ADR7678 ADR6428 ADR6436 ADR8052 ADR8051 ADR6426 ADR8055 ADR8104 ADR6464 ADR6887 ADR7252 ADR6724 ADR6886 ADR6722 ADR8421 ADR4006 ADR6387 ADR7144 ADR6448 ADR6387 ADR8063 ADR9047 ADR9056 ADR9059 ADR9050 ADR9053 ADR6387

S1A

S1B

S1C

S1D S1E

S1F

S1G-I S2A S2B

ESP1-3D PDS1-AID slk19∆ PDS1-AID wild-type esp1-2A esp1-3A esp1-1A esp1-2A+3A esp1-2A+1A esp1-3A+1A esp1-2A+3A+1A ESP1-18myc ESP1-3FLAG esp1-2A -3FLAG esp1-3A-3FLAG esp1-1A-3FLAG esp1-2A+3A-3FLAG esp1-2A+1A-3FLAG esp1-3A+1A-3FLAG esp1-2A+3A +1A-3FLAG wild-type wild-type esp1-2D esp1-1D ESP1-3D ESP1-2D+3D esp1-2D+1D ESP1-3D+1D ESP1-2D+3D+1D ESP1-18myc pds1∆ wild-type ESP1-18myc wild-type esp1-3A ESP1-18myc wild-type esp1-3A ESP1-3D SPC42-eGFP ESP1-3D SPC42-eGFP PDS1-AID pds1∆ [CEN-PDS1-URA3] pds1∆ esp1-1D [CEN-PDS1-URA3]

ADR6448 ADR8063 ADR4006 ADR6716 ADR6715 ADR6714 ADR6717 ADR6718 ADR6774 ADR6775 ADR5509 ADR5307 ADR5490 ADR5494 ADR5482 ADR5481 ADR5488 ADR5492 ADR5475 ADR5197 ADR4006 ADR5899 ADR5898 ADR5909 ADR5901 ADR5910 ADR5900 ADR5635 ADR5509 ADR5611 ADR4006 ADR5509 ADR4006 ADR6715 ADR5509 ADR4006 ADR6715 ADR6953 ADR4009 ADR6454 ADR6387 ADR6075 ADR6076

pds1∆ esp1-2D [CEN-PDS1-URA3] pds1∆ ESP1-3D [CEN-PDS1-URA3] pds1∆ esp1-2D+1D [CEN-PDS1-URA3] pds1∆ ESP1-3D+1D [CEN-PDS1-URA3] pds1∆ ESP1-2D+3D [CEN-PDS1-URA3] pds1∆ ESP1-2D+3D+1D [CEN-PDS1-URA3] S2C pds1∆ [CEN-PDS1-URA3] pds1∆ esp1-3A [CEN-PDS1-URA3] pds1∆ ESP1-3D [CEN-PDS1-URA3] S2D&E SPC42-eGFP pds1∆ SPC42-eGFP S3 PDS1-AID SPC42-eGFP ESP1-3D PDS1-AID SPC42-eGFP cdc55∆ PDS1-AID SPC42-eGFP cdc55∆ swe1∆ PDS1-AID SPC42-eGFP slk19∆ PDS1-AID SPC42-eGFP SPC42-eGFP ESP1-3D SPC42-eGFP cdc55∆ swe1∆ esp1-3A PDS1-AID SPC42eGFP swe1∆ cdc55∆ PDS1-AID CDC14-eGFP S4A SPC42-mCherry S4B wild-type spo12∆ cdc55∆ PDS1-AID spo12∆ cdc55∆ spo12∆ PDS1-AID cdc55∆ PDS1-AID spo12∆ cdc55∆ PDS1-AID S4C wild-type spo12∆ ESP1-3D PDS1-AID spo12∆ ESP1-3D spo12∆ PDS1-AID ESP1-3D PDS1-AID spo12∆ ESP1-3D PDS1-AID a See S1 Table for complete genotypes of each strain.

ADR6077 ADR6078 ADR6079 ADR6080 ADR6081 ADR6082 ADR6075 ADR9395 ADR6078 ADR4009 ADR4491 ADR6389 ADR6446 ADR6325 ADR7043 ADR8104 ADR4009 ADR6454 ADR7317 ADR7205 ADR22 ADR8097 ADR6436 ADR6428 ADR8110 ADR8109 ADR6426 ADR8113 ADR22 ADR8097 ADR6409 ADR6428 ADR8223 ADR8109 ADR6450 ADR8224