A total of 1,836 subjects (863 males and 973 females; aged 5-98 years) took part in the study and responded to the question on prevalence of dyspepsia.
the United States. We also detected a significant association of this SNP with dismotility. Conclusions: We found that C825T shows no significant association with FD under a dominant model, but it is significantly associated with FD when an additive model is assumed. Further studies with larger samples sizes are needed to validate our findings and to explore the potential mechanism underlying the association.
Sa1350
Introduction: SIBO is a condition in which large numbers of coliform gram negative bacteria (GNB) are present in the small bowel. Current "gold" standard for diagnosis of SIBO is jejunal aspiration and quantitative culture. Aim: 1) To determine the predictors of SIBO defined by GNB bacteria. 2) To compare results of quantitative culture versus molecular analysis. Methods: Prospective study was performed. Subjects were recruited from Motility Clinic with suspected SIBO. A standard prep protocol was followed prior to upper enteroscopy to proximal jejunum. Exclusion criteria were the use of colon cleansing prep, probiotics, or antibiotics in past 30 days. Detailed history and demographics were obtained. Specific risk factors for SIBO, diminished gastric acid exposure, and other potential factors were identified. Symptoms were assessed with Patient Assessment of GI Disorders-Symptom Severity Index (PAGY-SYM). Serum was obtained for IgA, anti-TTG IgA, iron saturation, B12, folate, INR, vitamin A, D and E levels. Methods were used to minimize contamination, and proximal jejunual luminal fluid was collected using a 180-cm aspiration catheter. SIBO was defined as >104 colony forming units cc of GNB by quantitative aerobic and anaerobic cultures. Biopsy of jejunum and stomach were obtained. Samples of aspirate, biopsy, and centrifuged serum were extracted for DNA and diluted at equal volume. 16S rRNA of global bacteria was amplified to estimate total # bacteria. Results: 23 subjects (mean age 47 yrs, 21 females) were enrolled. 13 subjects (57%) had established risk factors for SIBO, but only 9 (39%) had SIBO by culture. Predominant GNB cultured were E coli, Klebsiella, Serratia and Enterobacter. Results of predicting SIBO are presented in table. Established risk factors for SIBO was not a predictor of SIBO by cultures, but coexisting with reduced gastric acid was a significant predictor (odds ratio 7.3 (95th CI 1.1-48.3). There were no differences in lab evaluation, histology, total & subscale PAGY-SYM scores between subjects with and without SIBO. Figure shows the results of total 16S rRNA copies per μl between subjects with and without SIBO by culture. A trend of greater 16S rRNA copies in the luminal aspirate was seen in the SIBO subjects, but difference was not significant. 16S rRNA copies from mucosal biospy were similar between the two groups. 16S rRNA was identified from serum in only 2 subjects. Conclusions: 1) Established risk factors did not independently predict SIBO with coliform bacteria by cultures; however in combination with a reduction in acid exposure increased the risk for SIBO. 2) Subjects with SIBO by cultures tend to have greater total bacterial load by 16s rRNA copies. 3) We are currently analyzing the specific microbial compositions in all specimens using a deep V1-V5 and deep 16S rRNA PCR and sequencing approach. Table 1: Odds Ratio for predicting SIBO with coliform bacteria by quantitative culture
Sa1348 Prevalence and Socio-Demographic Determinants of Uninvestigated Dyspepsia in the Czech Republic Stanislav Rejchrt, Ilona Koupil, Marcela Kopacova, Bohumil Seifert, Miluska Skodova Fendrichova, Tomáš Douda, Jana Spirkova, Viktor Vorisek, Jan Bures, Ilja Tacheci Objectives Epidemiology of uninvestigated dyspepsia was studied in the Czech Republic for the first time in 2001 (1). The aim this current multi-centre prospective study was to evaluate dyspepsia using the same methods in a representative sample of general unselected population from the same geographical areas 10 years later. Methods A total of 22 centres entered the study. They were spread over the whole country, corresponding well to the geographical distribution of the Czech population. A total of 1,836 subjects (863 males and 973 females; aged 5-98 years) took part in the study and responded to the question on prevalence of dyspepsia. Complete data on variables used in our analysis was available for 1,685 subjects. The proportion of subjects reporting dyspepsia did not differ significantly between the restricted sample and the group excluded from multivariable analyses. Helicobacter pylori (Hp) status was investigated in all subject by means of 13C-urea breath test. Results In subjects aged 5-24 years, when we analyzed determinants of dyspepsia by type (subgroup A: dyspepsia as the only long-lasting symptom vs. subgroup B: dyspepsia as a part of the complex of other complaints or previously recognized diseases), we noted somewhat stronger increase in risk of dyspepsia A with age (OR 1.15 per 1 year of age, 95% CI 1.05-1.26, adjusted for gender) and with current use of antibiotics (OR 3.23 in users vs. non-users, 95% CI 0.87-11.9). In subjects aged 25+ years, when analyzed by type of dyspepsia, a statistically significant negative association of age with dyspepsia of type A became apparent (OR 0.95 per 1 year of age, 95% CI 0.94-0.97) while the association between age and dyspepsia type B was also statistically significant albeit positive (OR 1.02 per 1 year of age, 95% CI 1.01, 1.04). Furthermore, subjects who were single were at lower risk of dyspepsia type B (OR 0.46 in single vs. married, 95% CI 0.21-0.99, adjusted for age and gender). The unexpected protective effect of elementary education appeared also to be stronger for dyspepsia type B (OR for elementary vs. university educated 0.25, 95% CI 0.07-0.86, adjusted for age and gender). Hp negative subjects reported dyspepsia in 4.1% (aged 5-24 years) and 18.1 % (aged 25+ years). In Hp positive subjects, dyspepsia was present in 7.1% (aged 5-24 years; OR 1.45, 95% CI 0.41, 5.16) and 16.3% (aged 25+ years; OR 0.85, 95% CI 0.60, 1.21, adjusted for gender and age). Conclusions Despite the substantial decrease of Hp infection in the Czech Republic over the past 10 years, the prevalence and basic socio-demographic determinants of uninvestigated dyspepsia did not change significantly. Reference: Rejchrt et al. Eur J Gastroenterol Hepatol 2008; 20: 898-905. This study was supported by the research grant MH CZ NT115245/ 2010 and by the programme PRVOUK 3708. Sa1349 Impact of Water Load Test on the Gastric Myoelectric Activity in Experimental Pigs Ilja Tacheci, Jaroslav Kvetina, Michal Pavlik, Martin Kunes, Stanislav Rejchrt, Marcela Kopacova, Jan Bures INTRODUCTION: Surface electrogastrography (EGG) is a non-invasive method for the evaluation of gastric myoelectric activity. Porcine EGG is fully comparable with that recorded in healthy humans with normal rhythm of 3cycles per minute cpm. No data on EGG with water load test in experimental pigs were published so far. AIMS&METHODS: The aim of our experimental study was to evaluate the effect of different volume challenge on the gastric myoelectric activity. Five mature female pigs (Sus scrofa f. domestica, mean weight 30.9±1.6 kg) underwent the three EGG procedures within 3 weeks (using an Electrogastrography System MMS, Enschede, the Netherlands) under general anaesthesia. After a 15-minute baseline EGG was recorded, 23 °C warm water was administrated into the stomach by a gastric tube in different volumes: 500 mL (part A) or 1000 mL (part B) or 1500 mL (part C). A total of twenty 15-minute intervals were recorded afterwards. The results were expressed as running spectrum percent activity and the dominant frequency and amplitudes of slow waves were set. RESULTS: The dominant frequency of slow waves decreased from baseline values (3.34±0.69; 3.25±0.72; 3.30±0.68 cpm) to 2.97±0.52 (part A; p=0.004) or 3.16±0.48 (part B; p=0.116) or 3.09±0.28 (part C; p=0.013) 15 min. after intragastric water administration. Dominant frequency returned back to baseline values within 30 to 60 min. In the part A, the amplitudes of waves increased from basal values (2611±3726 μV^2) to their maximum after 15 min. (7733±1246; p=0.001), subsequently decreased to their minimum after 90 min. (312±283; p
