May 11, 2015 - [2] S. Dubiley, A. Ignatova, T. Mukhina, A. Nizova, S. Blagodatskikh, et al, Molecular epidemiology of tuberculosis · in the Tula area, Central ...
Prevalence of Latin-American-Mediterranean genetic family in population structure of Mycobacterium tuberculosis in Bulgaria Violeta Valcheva
a,* ,
Nalin Rastogi b, Igor Mokrousov
c,*
a
Department of Infectious Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria WHO Supranational Tuberculosis Reference Laboratory, Tuberculosis and Mycobacteria Unit, Institut Pasteur de Guadeloupe, Abymes 97183, Guadeloupe, France c Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, 197101 St. Petersburg, Russia b
A R T I C L E I N F O
A B S T R A C T
Article history:
Tuberculosis (TB) control remains an important public health priority for Bulgaria. The pop-
Received 30 March 2015
ulation structure of Mycobacterium tuberculosis is clonal and certain genetic families of this
Received in revised form
species (e.g., Latin-American-Mediterranean [LAM]) have attracted more attention due to
10 April 2015
their global dissemination and/or particular pathogenic properties, e.g., association with
Accepted 19 April 2015
multidrug resistance (MDR). The aim of this study was to evaluate the prevalence of the
Available online 11 May 2015
M. tuberculosis LAM family in Bulgaria based on the use of different molecular markers. A total of 101 previously spoligotyped M. tuberculosis strains were studied by LAM-
Keywords:
specific PCR assay to detect an insertion of IS6110 in the specific genome region. On the
Mycobacterium tuberculosis
whole, clear-cut results were obtained for most strains; spoligotype-based family was reas-
LAM (Latin-American-
signed in some of them. At the same time, double bands were amplified in some cases and
Mediterranean) family
warrant further validation studies of this method. The higher MDR rate among LAM versus
Genotyping
other genotype isolates was observed (P = 0.04). In conclusion, these results suggest a low
