The Canadian Society for Bioengineering The Canadian society for engineering in agricultural, food, environmental, and biological systems.
La Société Canadienne de Génie Agroalimentaire et de Bioingénierie La société canadienne de génie agroalimentaire, de la bioingénierie et de l’environnement
Paper No. CSBE16-XXX
Screening Ontario grown onion varieties for antioxidant properties Cynthya Maria Manohar BioNano Laboratory, School of Engineering, University of Guelph Guelph, Ontario, N1G 2W1, Canada.
Abdul Murayyan BioNano Laboratory, School of Engineering, University of Guelph Guelph, Ontario, N1G 2W1, Canada.
Suresh Neethirajan* BioNano Laboratory, School of Engineering, University of Guelph Guelph, Ontario, N1G 2W1, Canada.
Jun Xue Guelph Food Research Centre, Agriculture and Agri-Food Canada, N1G 5C9, Canada.
John Shi Guelph Food Research Centre, Agriculture and Agri-Food Canada, N1G 5C9, Canada.
Written for presentation at the CSBE/SCGAB 2016 Annual Conference Halifax World Trade and Convention Centre 3-6 July 2016
Papers presented before CSBE/SCGAB meetings are considered the property of the Society. In general, the Society reserves the right of first publication of such papers, in complete form; however, CSBE/SCGAB has no objections to publication, in condensed form, with credit to the Society and the author, in other publications prior to use in Society publications. Permission to publish a paper in full may be requested from the CSBE/SCGAB Secretary, 2028 Calico Crescent, Orleans, ON, K4A 4L7 or contact
[email protected]. The Society is not responsible for statements or opinions advanced in papers or discussions at its meetings.
ABSTRACT Agricultural by-products are in great demand in nutra-pharmaceutical and biomedical industries. Several studies have indicated that flavonoid based foods helps in reducing the incidence of cardiovascular diseases, breast cancer and diabetes. Onions are rich source of flavonoids and possess high levels of antioxidant activity. In our study, Ontario grown onion varieties namely Stanley, Safrane, Fortress, Lasalle and Ruby Ring were screened for their antioxidant properties. Here Low polarity water technology was used to extract the flavonoids from them and were identified by High Performance Liquid Chromatography. The free radical scavenging activities in the extracted samples were employed using 2,2-diphenyl-1picrylhydrazyl(DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and Ferric reducing ability of plasma (FRAP) assays. The total polyphenolic content was higher in the red onion variety, namely the Ruby Ring (1.69±0.15 mg/g, p