Selection and Properties of Escherichia coli Mutants Defective in the ...

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FRED TAYLOR AND JOHN E. CRONAN, JR.*. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510.
JOURNAL

OF

BACTERIOLOGY, Feb. 1976,

Vol. 125, No. 2 Printed in U.S.A.

p. 518-523

Copyright 0 1976 American Society for Microbiology

Selection and Properties of Escherichia coli Mutants Defective in the Synthesis of Cyclopropane Fatty Acids FRED TAYLOR AND JOHN E. CRONAN, JR.* Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510 Received for publication 3 November 1975

Mutants of Escherichia coli K-12 defective in the synthesis of cyclopropane fatty acids (CFA) have been selected and isolated by a L- [methyl-3H]methionine suicide procedure. Two mutants were isolated. Stationary-phase cultures of both mutants contain