FRED TAYLOR AND JOHN E. CRONAN, JR.*. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510.
JOURNAL
OF
BACTERIOLOGY, Feb. 1976,
Vol. 125, No. 2 Printed in U.S.A.
p. 518-523
Copyright 0 1976 American Society for Microbiology
Selection and Properties of Escherichia coli Mutants Defective in the Synthesis of Cyclopropane Fatty Acids FRED TAYLOR AND JOHN E. CRONAN, JR.* Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510 Received for publication 3 November 1975
Mutants of Escherichia coli K-12 defective in the synthesis of cyclopropane fatty acids (CFA) have been selected and isolated by a L- [methyl-3H]methionine suicide procedure. Two mutants were isolated. Stationary-phase cultures of both mutants contain