RESEARCH ARTICLE
Sepsis is associated with reduced spontaneous neutrophil migration velocity in human adults Steven L. Raymond1, Russell B. Hawkins1, Julie A. Stortz1, Tyler J. Murphy ID1, Ricardo Ungaro1, Marvin L. Dirain1, Dina C. Nacionales1, McKenzie K. Hollen1, Jaimar C. Rincon1, Shawn D. Larson1, Scott C. Brakenridge ID1, Frederick A. Moore1, Daniel Irimia2, Phil A. Efron1, Lyle L. Moldawer ID1*
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1 Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America, 2 Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America *
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Abstract OPEN ACCESS Citation: Raymond SL, Hawkins RB, Stortz JA, Murphy TJ, Ungaro R, Dirain ML, et al. (2018) Sepsis is associated with reduced spontaneous neutrophil migration velocity in human adults. PLoS ONE 13(10): e0205327. https://doi.org/ 10.1371/journal.pone.0205327 Editor: Raghavan Raju, Medical College of Georgia, Augusta, UNITED STATES Received: July 23, 2018 Accepted: September 24, 2018 Published: October 9, 2018 Copyright: © 2018 Raymond et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper. Funding: This work was supported by grants from the National Institute of General Medical Sciences (NIGMS) R01 GM-104481 (LLM), R01 GM040586 (LLM), R01 GM-097531 (SDL, LLM), R01 GM-113945 (PAE), and P50 GM-111152 (SCB, FAM, PAE, LLM). SLR was supported by a clinical research training fellowship awarded by the Surgical Infection Society Foundation. RBH and JAS were supported by a NIGMS post-doctoral
Sepsis is a common and deadly complication among trauma and surgical patients. Neutrophils must mobilize to the site of infection to initiate an immediate immune response. To quantify the velocity of spontaneous migrating blood neutrophils, we utilized novel microfluidic approaches on whole blood samples from septic and healthy individuals. A prospective study at a level 1 trauma and tertiary care center was performed with peripheral blood samples collected at
