EXCLI Journal 2019;18:1-7 – ISSN 1611-2156 Received: July 07, 2018, accepted: December 18, 2018, published: January 02, 2019
Original article: SEROMOLECULAR ASSESS OF TOXOPLASMA GONDII INFECTION IN PREGNANT WOMEN AND NEONATAL UMBILICAL CORD BLOOD Mohammad Menati Rashno1*, Shirzad Fallahi2, Zahra Arab-Mazar3, Hassan Dana1 1 2 3
*
Department of Biotechnology, Damghan Branch, Islamic Azad University, Damghan, Iran Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medial Sciences, Tehran, Iran Corresponding author: Mohammad Menati Rashno, Department of Biotechnology, Damghan Branch, Islamic Azad University, Damghan, Iran. Tel: +98-9384409404, E-mail:
[email protected]
http://dx.doi.org/10.17179/excli2018-1510 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).
ABSTRACT Toxoplasmosis is considered as one of the most prevalent human parasitic infections that can be transmitted from mother to the fetus. The onset of toxoplasmosis during pregnancy has clinical complications including spontaneous abortion, preterm labor, stillbirth and fetal abnormalities. The aim of this study was to investigate the prevalence of Toxoplasmosis infection in pregnant women and their infants in Lorestan province, Western Iran. Blood and sera samples were collected from 98 pregnant women and their infants. All collected samples were examined for Toxoplasma gondii infection by serological tests (ELISA IgM & IgG) and PCR assay. Among the 98 samples of mother and umbilical cord prevalence of anti-Toxoplasma IgG, was 34/98 (34.69 %) and 33/98 (33.67 %), respectively. All pregnant women were negative for, anti-Toxoplasma IgM while it was found in 5/98 (5.1 %) of umbilical cords. Based on PCR analysis, Toxoplasma infection was detected in 5 (5.1 %) and 7 (7.14 %) of mother and umbilical cords, respectively. Molecular test along with evaluation of IgM (P
