Serum ARID1A acts as a potential diagnostic biomarker for gastric ...
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Serum ARID1A acts as a potential diagnostic biomarker for gastric ...
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Original Article Serum ARID1A acts as a potential diagnostic biomarker for gastric cancer Xudong Gao1, Xiaodong Tan1, Ying Zeng2, Zhenkun Wang1, Qihan Zhao1 School of Public Health, Wuhan University, Wuhan, China; 2School of Medicine, University of South China, Hengyang, China 1
Received February 15, 2016; Accepted July 5, 2016; Epub October 1, 2016; Published October 15, 2016 Abstract: Background: AT-rich interactive domain 1A gene (ARID1A) had been reported to act as a tumor suppressor in gastric cancer (GC), and reduced expression of ARID1A is significantly correlated with lymphatic invasion and lymph node metastasis. However, its diagnostic value in GC remains unknown. The aim of this study was to detect the expression of serum ARID1A and investigate its diagnostic value in GC. Methods: ARID1A mRNA and protein expression were assessed in 120 GC serum samples and 70 healthy control samples by quantitative real-time polymerase chain reaction (qRT-PCR) and ELISA, respectively. And the association between ARID1A expression and clinicopathologic parameters was evaluated by Chi-square test. To detect the diagnostic value of serum ARID1A, receiver operating characteristic (ROC) curve were established. Results: The expression of ARID1A was down-regulated in GC serum compared to that in healthy controls both at mRNA and protein level (P
