Serum Vitamin D Levels in Patients with Acute

Ocular Immunology and Inflammation

ISSN: 0927-3948 (Print) 1744-5078 (Online) Journal homepage: http://www.tandfonline.com/loi/ioii20

Serum Vitamin D Levels in Patients with Acute Anterior Uveitis Zeynep Dadaci MD, Servet Cetinkaya MD, Nursen Oncel Acir MD, Mufide Oncel MD & Mehmet Borazan MD To cite this article: Zeynep Dadaci MD, Servet Cetinkaya MD, Nursen Oncel Acir MD, Mufide Oncel MD & Mehmet Borazan MD (2016): Serum Vitamin D Levels in Patients with Acute Anterior Uveitis, Ocular Immunology and Inflammation, DOI: 10.3109/09273948.2016.1139735 To link to this article: http://dx.doi.org/10.3109/09273948.2016.1139735

Published online: 22 Mar 2016.

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Date: 25 March 2016, At: 14:09

Ocular Immunology & Inflammation, 2016; 00(00): 1–5 © Taylor & Francis Group, LLC ISSN: 0927-3948 print / 1744-5078 online DOI: 10.3109/09273948.2016.1139735

ORIGINAL ARTICLE

Serum Vitamin D Levels in Patients with Acute Anterior Uveitis Zeynep Dadaci,

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1

MD

1

, Servet Cetinkaya, MD2, Nursen Oncel Acir, Mehmet Borazan, MD1

MD

1

, Mufide Oncel,

MD

3

, and

Mevlana University School of Medicine, Department of Ophthalmology, Konya, Turkey, 2Ophthalmology Clinics, Turkish Red Crescent Hospital, Konya, Turkey, and 3Mevlana University School of Medicine, Department of Clinical Biochemistry, Konya, Turkey

ABSTRACT Purpose: To evaluate serum 25-hydroxyvitamin D levels in patients with acute anterior uveitis (AAU). Methods: This observational case–control study involved 20 patients with AAU, and 20 consecutive, age and sexmatched healthy subjects without any ocular or systemic diseases. Serum 25-hydroxyvitamin D was quantified with electrochemiluminescence technique. Results: No significant differences were found between the groups with respect to age (p = 0.185) and sex (p = 0.465). Serum vitamin D levels of the subjects with AAU (mean 5.75 ± 4.50 ng/mL, median 4.00 ng/mL, range: 3.00–19.00 ng/mL) were significantly lower than the control group (mean 12.96 ± 5.89 ng/mL, median 11.00 ng/ mL, range: 5.20–25.92 ng/mL) (p30 cells).

Biochemical Analysis METHODS

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Study Population This prospective, observational, case–control study comprised of 40 subjects (20 patients with idiopathic or HLA-B27-associated AAU and 20 control subjects). All patients were recruited in the study between May and October 2015, and evaluated at the Mevlana University and Turkish Red Crescent eye clinics. Patients with signs and symptoms of acute anterior uveitis (conjunctival hyperemia, pain, photophobia, flare, and anterior chamber cells) without any evidence of posterior segment inflammation were included in the AAU group. Patients with granulomatous, infective or traumatic uveitis, and known uveitic syndromes were excluded. The control group was composed of 20 consecutive subjects of similar age (18–67 years) and gender as the study group, with normal ocular examination, except refractive disorders, without any history of systemic diseases, drug or dietary supplement consumption. To minimize cultural influences or geographic effects on the findings, the study was conducted in the central region of Turkey, and all participants lived in the same region. The research protocol was approved by the Mevlana University Ethics Committee (2015/154). The study was performed in compliance with good clinical practice guidelines and in accordance with the tenets of the Declaration of Helsinki. Written informed consent was obtained from each patient prior to enrollment into the study. Pregnant or breast-feeding women were excluded from the study. Subjects with any ocular pathology other than AAU, such as allergic conjunctivitis and dry eye syndrome, were also excluded. Subjects who were smokers and who had a history of consumption of vitamin supplements or alcohol during the 4 weeks prior to the study, and obese patients, were also excluded. The associated systemic diseases and the drugs of the patients with AAU were noted and patients were consulted with internists for systemic evaluation and vitamin D supplementation. All subjects underwent a full ophthalmologic examination, including: best-corrected visual acuity (BCVA) obtained with a Snellen projection chart; refraction; slitlamp biomicroscopy; intraocular pressure (IOP) measurement with Goldmann applanation tonometry; and dilated fundoscopy with a 90 Diopter lens. The intensity

Fasting venous blood sample was obtained from each subject from the antecubital vein into the serum separator tubes. After centrifugation at 2000 g for 15 min serum samples were aliquoted, labeled and stored at –20ºC for a maximum of 30 days until analysis. When we reached the targeted sample size, serum samples were thawed and serum 25-hydroxyvitamin D levels were quantified with electrochemiluminescence technique on Modular Analytics E 170 analyzer (Roche Diagnostics, GmbH, Germany). Measuring range for 25-hydroxyvitamin D was 3–70 ng/mL (7.5–175 nmol/L). Between run precision values determined with analysis of serum pools were as follows: 13.1% for low and 3.4% for high concentrations of 25hydroxyvitamin D, respectively.

Statistical Analysis Statistical analyses were performed using a statistical program (SPSS version 15.0, SPSS Science, Chicago, IL). The distribution of the numeric data was tested with the Shapiro–Wilk test. The Mann–Whitney Utest was used for testing the difference between vitamin D levels for both patient and control groups. The mean age of groups were compared with the Mann– Whitney U-test and the distribution of sex between the groups was compared using the χ2-test with continuity correction. All p values