short bouts of mechanical loading are as effective as ... - CiteSeerX
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short bouts of mechanical loading are as effective as ... - CiteSeerX
Cell-seeded scaffolds were compressed at 5% global strain ... 2 Kroto Research Institute, Department of Materials Science and Engineering, University of ...
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ISSN 1473-2262 Load vs. Dex induced matrix production by hMSCs
SHORT BOUTS OF MECHANICAL LOADING ARE AS EFFECTIVE AS DEXAMETHASONE AT INDUCING MATRIX PRODUCTION BY HUMAN BONE MARROW MESENCHYMAL STEM CELLS A. Sittichokechaiwut1, J.H. Edwards2, A.M. Scutt3, and G.C. Reilly2*
2
1 Department of Preventive Dentistry, Naresuan University, Thailand Kroto Research Institute, Department of Materials Science and Engineering, University of Sheffield, U.K. 3 School of Medicine and Biomedical Sciences, University of Sheffield, U.K.
Abstract
Introduction
Dexamethasone (Dex) is used widely to induce differentiation in human mesenchymal stem cells (hMSCs); however, using a pharmaceutical agent to stimulate hMSC differentiation is not the best choice for engineered tissue transplantation due to potential side-effects. The goal of the present study was to investigate the effects of dynamic compressive loading on differentiation and mineralized matrix production of hMSCs in 3D polyurethane scaffolds, using a loading regimen previously shown to stimulate mineralised matrix production of mature bone cells (MLOA5). hMSCs were seeded in polyurethane scaffolds and cultured in standard culture media with or without Dex. Cell-seeded scaffolds were compressed at 5% global strain for 2 h on day 9 and then every 5 days in a media-filled sterile chamber. Samples were tested for mRNA expression of alkaline phosphatase (ALP), osteopontin (OPN), collagen type 1 (col 1) and runt-related transcription factor2 (RUNX-212 h) after the first loading, cell viability by MTS assay and alkaline phosphatase activity at day 12 of culture and cell viability, collagen content by Sirius red and calcium content by alizarin red at day 24 of culture. Neither Dex nor loading had significant effects on cell viability. Collagen content was significantly higher (p
