significant correlations - PLOS

P motifs

S motifs

A

C

6 T T N N S N T S S S R T N A G H H N F G G S S T H G N N A M N T S C I

1’ D N D N N S T S R C D S R D D M S C G H S G L P V N T G N D E R D S D

A 2 23 7 6 10 1 0 5 0 2 0 3 0 0 0 0 0 0 1 1 1 1 1 1 2 0 1 1 2 0 0 1 4 0 1

C 0 0 22 46 1 12 0 1 0 0 0 0 2 0 1 0 0 5 0 0 0 0 0 0 0 1 8 2 2 1 0 0 3 1 0

G 23 2 7 3 0 0 3 0 2 0 0 0 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 3 0 1

U 1 0 68 31 1 3 0 0 0 0 3 1 0 1 0 2 2 3 0 0 0 0 0 0 1 0 8 5 0 0 1 0 1 2 1

p-value 9.70E-18 1.34E-14 2.09E-09 2.39E-06 3.42E-06 1.50E-04 5.59E-04 1.02E-03 4.84E-03 1.21E-02 1.38E-02 2.01E-02 2.63E-02 3.56E-02 3.56E-02 4.43E-02 4.43E-02 5.06E-02 7.62E-02 7.62E-02 7.62E-02 7.62E-02 7.62E-02 7.62E-02 8.66E-02 1.16E-01 1.19E-01 1.35E-01 1.96E-01 2.22E-01 2.22E-01 2.22E-01 2.34E-01 2.88E-01 3.89E-01

6 T S S T N C N N G A T C G P A S T T S T L N A F I P S T V K N A T A T N

1’ D D N N D N T N S R H D D C H E E K K L D E T N N N S Q S D H D S N T S

A 9 1 20 18 5 3 0 5 2 0 1 0 0 0 1 1 1 1 2 2 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2

C 2 1 2 1 13 0 12 8 0 2 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 2 2 0 3 0 1 4

G 27 13 1 2 5 0 3 9 0 0 2 1 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 1 1

U 4 1 5 3 44 0 4 5 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 1 1 1 1 1 0 1 1 1 1 0 5

p-value 4.72E-14 1.16E-09 8.69E-09 1.25E-08 1.20E-06 2.13E-03 2.14E-03 9.80E-03 1.21E-02 2.63E-02 3.56E-02 4.64E-02 4.64E-02 4.64E-02 7.62E-02 7.62E-02 7.62E-02 7.62E-02 8.66E-02 8.66E-02 1.16E-01 1.16E-01 1.55E-01 1.55E-01 1.55E-01 1.55E-01 1.55E-01 1.55E-01 1.55E-01 1.79E-01 1.79E-01 2.22E-01 2.83E-01 3.60E-01 3.89E-01 7.06E-01

D

B 6 T N S G C R F A I M H

R 59 27 28 4 0 0 1 4 2 1 2

Y 2 216 7 2 3 3 0 3 1 1 5

p-value 1.16E-25 4.10E-17 3.36E-08 1.09E-01 1.11E-01 1.11E-01 1.45E-01 2.22E-01 2.57E-01 6.53E-01 7.38E-01

6 T S N C A G K P F I L V

R 67 39 30 4 3 3 1 1 0 0 0 0

Y 17 10 99 0 5 0 2 1 1 1 1 1

p-value 2.72E-16 4.24E-10 4.32E-08 4.55E-02 8.33E-02 8.33E-02 3.17E-01 3.17E-01 1.00E+00 1.00E+00 1.00E+00 1.00E+00

Table S3. Correlations between amino acids at positions 6, 1’ and aligned nucleotides. The tables show frequencies of co-occurrence of amino acids and nucleotides from the alignments in Fig. 2 and Fig. S1. A: P motifs, positions 6, 1’ versus each nucleotide; B: S motifs, positions 6, 1’ versus each nucleotide; C: P motifs, position 6 versus purines (R), pyrimidines (Y); D: S motifs, position 6 versus purines (R), pyrimidines (Y). P-values were calculated using G-tests. P-values in A and B are for the most positively correlated nucleotide. Significance was evaluated at 5% allowing for multiple testing (using the Šidák correction). Green shading indicates significantly correlated, magenta shading indicates significantly anti-correlated.