Simultaneous Direct Counting of Total and Specific Microbial Cells in ...
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Simultaneous Direct Counting of Total and Specific Microbial Cells in Seawater, Using a Deep-Sea Microbe as Target AKIHIKO MARUYAMA1*
AND
MICHINARI SUNAMURA1,2
Bioconsortium Analysis Program, National Institute of Bioscience and Human Technology, Higashi, Tsukuba, Ibaraki 305-8566,1 and Mitsubishi Kasei Institute of Life Science, Machida, Tokyo 194-8511,2 Japan Received 1 November 1999/Accepted 2 March 2000
To rapidly and accurately enumerate total and specific microbes in aquatic samples, fluorescent in situ hybridization was combined with direct counting via direct immobilization of cells on a polymer-coated Nuclepore filter. The technique, named FISH-DC, achieved almost complete recovery of total cells and reproducibility of Psychrobacter pacificensis cells of deep-sea origin (error,
