May 9, 2012 - Abstract. In order to investigate the effects of RNA interfer- ence of decoy receptor 3 (DcR3) on the sensitivity of gastric cancer cells to ...
EXPERIMENTAL AND THERAPEUTIC MEDICINE 4: 465-468, 2012
siRNA targeting decoy receptor 3 enhances the sensitivity of gastric carcinoma cells to 5-fluorouracil XIAO-TAO XU, ZE-ZHANG TAO, QI-BIN SONG, YI YAO and PENG RUAN Department of Oncology, Renmin Hospital, Wuhan University, Hubei 430060, P.R. China Received March 15, 2012; Accepted May 9, 2012 DOI: 10.3892/etm.2012.606 Abstract. In order to investigate the effects of RNA interference of decoy receptor 3 (DcR3) on the sensitivity of gastric cancer cells to 5-fluorouracil (5-FU) and the relevant mechanisms, siRNA against DcR3 was transfected into the gastric cancer cell line AGS. AGS cells were treated with different doses of 5-FU or for different time periods. The sensitivity of AGS cells to 5-FU was determined. The cell survival rate was detected by MTT assay. The apoptotic rate was determined by DAPI staining, and the expression of related proteins were detected by western blot analysis. The results showed that the cell survival rate was significanlty decreased in the knockdown group compared to the control group at different doses of 5-FU (P
