Tumor-released RNA may mediate intercellular communication and serve as biomarkers. Here we develop a ...... miR-615-3p .... which also serves as a precursor for miR-124631, and specific .... (ANG), a member of ribonuclease A family44.
... of Rochester, Rochester, New York 14627, USA; 3Department of Chemistry and Center for Research ... Supplemental material is available at http://www.genesdev.org. .... Mics and the parental Mac are destroyed (5); and cells resume.
1A [stage 5], J,K). Then, it disappeared before DNA elimination occurred. (â¼14 h post-mixing) (Fig. 1L). Similar transitions of local- ization from the old Mac to the ...
Jan 26, 2015 - VOLUME 16 NUMBER 3 MARCH 2015 NATURE IMMUNoloGy ... Downregulation of linc-MAF-4 skewed T cell differentiation toward the TH2 ...
Saakshi Jalali1,2, Shrey Gandhi1 and Vinod Scaria1,2*. Abstract ...... Jalali S, Kapoor S, Sivadas A, Bhartiya D, Scaria V. Computational approaches towards ...
Aug 31, 2016 - EVs were isolated from cell culture media using the Total Exosome ..... Proteomics, transcriptomics and lipidomics of exosomes and ecto-.
Sep 23, 2015 - Overview of the RNA sequencing (RNA-seq) workflow, including filtering and ... against the mouse genome with an E-value cutoff of 1e 10. Any hits ...... F, Abdelhamid RF, Alioto T, Antoshechkin I, Baer MT, Bar NS, Batut.
Aug 1, 2018 - 45 samples were used to construct WGCNA network. First ..... The recent single-cell RNA-Seq (scRNASeq) technology makes it possible to ...
Jan 20, 2016 - 1RNA Biology Group, Institute for Molecular Infection Biology (IMIB), University of Wu¨ rzburg, Josef-Schneider-Strasse 2, 97080 Wü rzburg,.
terization of a novel small non-coding RNA: NrsZ ... Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
May 9, 2016 - found that the EVs contained predominantly a 22 base miR-224 and to a lesser extent a ... 224 versus the bovine 23 base annotated sequence.
Sep 5, 2013 - a double-stranded miRNA molecule. ... target of the miRNA, miRNAs do not have a high specificity for their ... of siRNA-mediated mRNA degradation, based on the observation that ... suggesting the cooperation between mir-17-19b and c-Myc
Jul 7, 2015 - served than mRNA and small noncoding RNAs [4, 5], lack of conservation does not ... associated noncoding RNAs dynamically expressed in ESCs during cardiac ... In human, several cytoplasmic lncRNAs transactivate Staufen1- ..... noncoding
Sep 2, 2014 - Nasopharyngeal Carcinoma and Their Prognostic Value ... Nasopharyngeal Carcinoma, Sun Yat-Sen University Cancer Center, 651 Dongfeng ...
Jul 15, 2014 - the entire plant life cycle (1). One of the ... are an important class of. ncRNAs that recently have been shown to possess a diverse set of.
Oct 26, 2010 - Envelope Reverse 1 (SER1), and SARS Spike Reverse 4 (SSR4) and those with primers designed for mouse 18S gene using the same strains ...
Feb 15, 2017 - many highly-connected genes, including XLOC_058593, BMP3, MYOD1, and LAMP3. This study provides a valuable resource for chicken ...
Aug 26, 2013 - Minghui Zhu,1 Guoyong Chen,1 WeiWei Wang,1 Fred G. Biddle,4 and Jianqin Gu3. 1 Clinical Research Center, People's Hospital of ...
The methylation status of the MEG3 promoter in cervical cancer tissue samples was ..... ferentiation, and lymphatic vascular space invasion (LVSI). Univariate ...
Dec 13, 2017 - hans). β-actin was used ..... 21 Knauss JL, Sun T: Regulatory mechanisms of long noncoding RNAs in vertebrate central nervous system.
May 18, 2018 - aHoward Hughes Medical Institute, The Rockefeller University, New ... Hadassah–Hebrew University Medical Center, Jerusalem 91120, ...... (Zymo Research Corporation), followed by vacuum-applied washes with 970 μL.
May 18, 2018 - sues such as liver (2), placenta (3), endocrine organs (4, 5), or .... with the denaturant or extracting agent before the biofluid was ... (SI Text: Method Optimization of exRNA and exDNA Isolation and. Fig. ... RNA & DNA isolation ...
Oct 5, 2015 - 1IBERS, Aberystwyth University, Aberystwyth, UK; 2Centre for Proteomics and ... Leiden University Medical Center, Leiden, The Netherlands.
Oct 15, 2013 - Wheel Projections program Version: Id: wheel.pl,v 1.4. 2009-10-20 .... yeast mitochondrial ORFs utilize the UAA stop codon (Figure. 4). Both the ...
Supplemental 4 Original western blot images for all samples. Samples unmarked and unrelated to this project are also in blots for CD90, HSP70 and TSG101.
Small non-coding RNA landscape of extracellular vesicles from human stem cells Sippy Kaur, Ahmed G Abu-Shahba, Riku O Paananen, Heidi Hongisto, Hanna Hiidenmaa, Heli Skottman, Riitta Seppänen-Kaijansinkko, Bettina Mannerström Supplemental 1 Characterisation for human PSC-2. A) Human PSC-1 characterized for A) typical undifferentiated colony morphology in phase contrast image and B) expression of pluripotency markers Nanog, OCT-3/4, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81, and lack of expression of early differentiation marker SSEA-1 after immunofluorescence staining. Corresponding nuclei stains with DAPI shown. C) The results of the KaryoLite BoBs assay are shown as signal relative to karyotypically normal female (/F, red) and male (/M, blue) genomic DNA used as a reference (equal to 1) for each of the probes covering both p and q arms of all chromosomes. Software threshold for changes shown as a green lines and deviations in red. D) Pluripotency shown after spontaneous differentiation in vitro as expression of markers for mesoderm, endoderm, and ectoderm. All scale bars 200 µm.
Supplemental 4 Original western blot images for all samples. Samples unmarked and unrelated to this project are also in blots for CD90, HSP70 and TSG101.
Calnexin
CD63
CD90
Hsp70
TSG101
Supplemental 5 miRNA Phred quality scores and insert length distributions for all the samples in the study.