MAT2B, MTHFR, MTHFD1, MTR, MTRR, and TYMS. SNP genotyping was car- ried out using a high throughput SNPlex genotyping platform based on multiplex-.
SMFM Abstracts
www.AJOG.org 573
REGULATION OF MATERNAL FEEDING DURING LACTATION PERIOD MAY CONTROL ADULTHOOD HYPERTENSION HIDENORI TAKAHASHI1, TOSHIAKI OKAWA2, KEIYA FUJIMORI3, AKIRA SATO4, 1Fukushima Med. Univ., Fukushima, Fukushima, Japan, 2 Fukushima Medical University, Dept. Ob/Gyn, Fukushima, Fukushima, Japan, 3 Fukusima Meidical University, Obstetrics and Gynecology, Fukushima, Fukushima, Japan, 4Fukushima Medical University, Obstetrics and Gynecology, Fukushima, Fukushima, Japan OBJECTIVE: Exposure to undernutrition during fetal life has been proposed as an underlying cause of adult hypertension, but the effect of either high fat nourishment or undernutrition during lactation period on blood pressure is unclear. Our objective was to investigate the most effective maternal nourishment and feeding period for offspring induced adulthood hypertension in using high-fat diet (HFD). STUDY DESIGN: We use 5 types pregnant Wistar rats as fed with normal nutrition (Group A), nutritionally restricted by feeding with 30% of the normal gestation-matched dietary intake from day 17 of gestation to delivery (Group B), 30% restricted after delivery to the end of lactation period (Group C), with a high fat diet (HFD) during gestation to lactation period (Group D) and with HFD nutritionally 30% restricted from the day of delivery to the end of lactation period (Group E). The offspring was measured Body Weight (BW) and measured blood pressure at 12, 24 and 60 weeks by using indirect tail-cuff method. Statically analysis was performed using one-way ANNOVA. RESULTS: BW was significantly reduced in B offspring compared to another (A, C, D, E) male offspring at day 1 (p⬍0.01). At day 28 after delivery, BW was significantly reduced in C, E offspring compared to A, D in male offspring (p⬍0.01). At 12 weeks old, BW of all type offspring was no difference. Systolic and Diastolic blood pressures were significantly elevated at 12 and 60 weeks in offspring of D⬎ E ⬎ B ⬎ C ⬎A. (p⬍0.01, vs. A). At 24 weeks, hypertensive offspring as B⬎D⬎E⬎C⬎A. (p⬍0.01, vs. A) CONCLUSION: Maternal high fat environment make a hypertensive offspring, but regulation of fat feeding during lactation period may reduce adulthood hypertension. In case with normal food, restrictive feeding during late gestation is more effective than lactation period for inducing hypertensive male offspring. Regulation of maternal feeding not only during late gestation but also lactation period may control adulthood hypertension.
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0002-9378/$ - see front matter doi:10.1016/j.ajog.2008.09.603
RESULTS OF A GENETIC ASSOCIATION STUDY OF GENES IN FOLATE METABOLISM AND SPINA BIFIDA CARLA A. MARTINEZ1, HOPE NORTHRUP2, JONE-ING LIN3, ALANNA MORRISON3, JACK M. FLETCHER4, GAYLE TYERMAN5, KIT SING AU2, 1University of Texas Health Science Center at Houston, Obstetrics, Gynecology, and Reproductive Sciences, Houston, Texas, 2University of Texas Health Science Center at Houston, Pediatrics, Houston, Texas, 3University of Texas School of Public Health, Human Genetics Center, Houston, Texas, 4University of Houston-Texas Medical Center Annex, Department of Psychology, Houston, Texas, 5Shriners Hospitals for Children, Los Angeles, California OBJECTIVE: Neural tube defects (NTDs) are one of the most common birth defects in the United States. Epidemiologic studies have shown that maternal folate status is foremost in the prevention of occurrence and recurrence of many NTDs. Our objective was to test single nucleotide polymorphisms (SNPs) of 11 candidate genes known to regulate the folate/homocysteine metabolism pathway for their contribution to spina bifida (SB) susceptibility. STUDY DESIGN: The study population consisted of 608 (227 Caucasians, 331 Hispanics of Mexican descent, 50 of other ethnic background) unrelated children with SB. Blood samples were obtained from patients and their parents with extraction of genomic DNA. We investigated 48 SNPs located in the coding sequence or promoter region of 11 candidate genes which were predicted to have putative functional significance. These genes included: AHCY, BHMT, CBS, DHFR, MAT2A, MAT2B, MTHFR, MTHFD1, MTR, MTRR, and TYMS. SNP genotyping was carried out using a high throughput SNPlex genotyping platform based on multiplexoligonucleotide ligation/PCR assay. Genotypes generated were on simplex cases (triads of an affected and parents; duos of an affected and one parent) and analyzed by transmission disequilibrium test (TDT) for genetic associations between transmission of alleles and SB in the offspring. RESULTS: A statistically significant association of SNP rs5742905 in CBS (T allele, frequency 0.99), rs1643649 in DHFR (T allele, frequency 0.74-0.8) and rs2853533 in TYMS (G allele, frequency 0.98) was found (p⫽ 0.015, 0.041, and 0.021, respectively). These SNPs were in Hardy-Weinberg equilibrium. CONCLUSION: In our study, 3 SNPs not previously studied on the CBS, DHFR and TYMS genes are associated with increased susceptibility to SB. Interaction of risk alleles between genes may contribute to dys-regulated folate/homocysteine metabolism subsequently increasing SB susceptibility. 0002-9378/$ - see front matter doi:10.1016/j.ajog.2008.09.605
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POSTPARTUM LEVELS OF TOTAL CHOLESTEROL AND TRIGLYCERIDES IN WOMEN WITH AND WITHOUT GESTATIONAL DIABETES MELLITUS AND PREECLAMPSIA VICTOR NOVACK1, LENA NOVACK2, ARNON WIZNITZER3, 1Harvard Clinical Research Institute, Boston, Massachusetts, 2Ben-Gurion University of the Negev, Faculty of Health Sciences, Epidemiology, Beer-Sheva, Israel, 3Division of Obstetrics and Gynecology, Soroka University Medical Center, Beer-Sheva, Israel OBJECTIVE: A number of diseases that affect the cardiovascular system emerge during pregnancy. We hypothesized that gestational diabetes (GDM) and preeclampsia can be associated with elevated postpartum levels of lipids based on the common factor of insulin resistance. OBJECTIVE: Assessment of association between GDM and preeclampsia and postpartum levels of total cholesterol (TC) and triglycerides (TG). STUDY DESIGN: Data regarding 2,821 women without cardiovascular co-morbidities (atherosclerotic diseases, hypertension, and diabetes mellitus) with lipids assessed within 12 months after the delivery were evaluated (closest pair of delivery-lipid test was chosen). GDM and preeclampsia were diagnosed based on universal screening. RESULTS: Overall, 406 women (14.4%) had one of the conditions: GDM was diagnosed in 187 women (6.6%), and preeclampsia in 240 women (6.6%). This group was slightly older (30.8 vs. 29.5 years, p⬍0.001), but had the same gravidity and parity as the rest of the cohort (average 4.4 vs. 4.2 and 3.7 vs. 3.7). Following the delivery levels of TG and TC rapidly declined to plateau in 3 months (Figure 1). In women with GDM or preeclampsia levels of both lipids remained higher throughout 12 postpartum months.
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DO THE SECOND TRIMESTER AMBULATORY 24-HOUR, AWAKE AND SLEEP BLOOD PRESSURE MEASUREMENTS CORRELATE WITH PLACENTAL WEIGHT IN WOMEN AT RISK FOR HYPERTENSION IN PREGNANCY? CORENTHIAN BOOKER1, SERDAR URAL1, ALLEN KUNSELMAN2, WILLIAM DODSON1, JOHN REPKE1, RICHARD LEGRO1, 1Pennsylvania State University, Hershey, Pennsylvania, 2Penn State College of Medicine, Department of Public Health Sciences, Hershey, Pennsylvania OBJECTIVE: Our aim was to assess the correlation between placental weight and the average 24-hour ambulatory blood pressure measurement (24-ABPM), awake (A-ABPM) and sleep (S-ABPM) in patients at risk for hypertension (HTN) in pregnancy. To our knowledge this is the first study attempting to analyze these relationships. STUDY DESIGN: This prospective study observed 21 pregnant women with at least one risk factor for HTN in pregnancy between 14-24 weeks gestation. Using an ABP monitor BP measurements were collected every thirty minutes for twenty four hours. Placental weights (corrected for gestational age) were compared with the mean systolic (SBP), diastolic (DBP) and mean arterial pressure (MAP) ABPM parameters. RESULTS: Using Spearman’s correlation,the 24-ABPM SBP and MAP and the S-ABPM SBP, DBP, and MAP negatively correlated with the placental weights. CONCLUSION: Our study demonstrates that placental weight negatively correlates with the 24-ABPM and S-ABPM but not the A-ABPM. Further studies are necessary to determine whether these measurements are clinically useful tools in this patient population. 24-ABPM, S-ABPM and A-ABPM Negatively Correlates with Placental Weight.
CONCLUSION: During 12 postpartum months levels of TC and TG are higher in group of women with GDM or preeclampsia. This finding that can indicate that dyslipidemia and these gestational morbidities may have a common pathophysiological mechanism. Postpartum lipids levels should be monitored in women with gestational diabetes mellitus and preeclampsia.
Placental Weight (g) vs.
r
95% CI
p value
24-ABPM SBP 24-ABPM DBP 24-ABPM MAP A-ABPM SBP A-ABPM DBP A-ABPM MAP S-ABPM SBP S-ABPM DBP S-ABPM MAP
⫺0.49 ⫺0.53 ⫺0.52 ⫺0.38 ⫺0.39 ⫺0.37 ⫺0.64 ⫺0.56 ⫺0.57
⫺0.76, ⫺0.08 ⫺0.78, ⫺0.13 ⫺0.78, ⫺0.11 ⫺0.70, 0.06 ⫺0.71, 0.05 ⫺0.69, 0.07 ⫺0.84, ⫺0.27 ⫺0.80, ⫺0.16 ⫺0.79, ⫺0.07
0.0222 0.0121 0.0146 0.0916 0.0776 0.0954 0.0146 0.0092 0.0070
0002-9378/$ - see front matter doi:10.1016/j.ajog.2008.09.606
0002-9378/$ - see front matter doi:10.1016/j.ajog.2008.09.604
Supplement to DECEMBER 2008 American Journal of Obstetrics & Gynecology
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