IOP Conference Series: Materials Science and Engineering
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Spider Silk Processing for Spidroin Recovery from Crossopriza Lyoni Web To cite this article: J A Mohtar et al 2018 IOP Conf. Ser.: Mater. Sci. Eng. 318 012016
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MUCET 2017 IOP Publishing IOP Conf. Series: Materials Science and Engineering 318 (2018) 012016 doi:10.1088/1757-899X/318/1/012016 1234567890‘’“”
Spider Silk Processing Crossopriza Lyoni Web
for
Spidroin
Recovery
J A Mohtar1, W L Ooi2, and F Yusuf3 1 Department of Chemical Engineering Technology, Technology, Universiti Malaysia Perlis, Uniciti Alam Chuchuh, Padang Besar, Perlis, Malaysia 2 Department of Chemical Engineering Technology, Technology, Universiti Malaysia Perlis, Uniciti Alam Chuchuh, Padang Besar, Perlis, Malaysia 3 Department of Biotechnology Engineering, Kulliyah of Islamic University Malaysia, Gombak, Malaysia
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Faculty of Engineering Campus, 02100 Sungai Faculty of Engineering Campus, 02100 Sungai Engineering, International
E-mail:
[email protected] Abstract. Spider silk is a potential biomaterial that can be used in various applications for its outstanding physicomechanical properties attributed by the spidroin composition. Efforts for commercializing spider silks have been mainly focused on the characterization of spidroins from the Entelegyne spiders for exceptional fibre construction. Hence, studies on silk proteins from the Haplogyne species remain neglected. The aim of this study is to isolate spidroin from Crossopriza lyoni web. Silk processing involved the pretreatment of fibres for the shell layer removal from the surface. A screening study was conducted to analyze the effect of temperature, incubation time and agitation speed on spidroin extraction using Ajisawa’s reagent by OFAT analysis followed by statistical optimization of the extraction process via RSM for maximal protein recovery. All parameters exerted significant effect on spidroin recovery (p
