Study The Effect of Purified Goat Milk Lactoferrin on ...
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Study The Effect of Purified Goat Milk Lactoferrin on ...
Lactoferrin is an important protein in many biological applications as a ... tion for the cytotoxic effect of the purified goat milk lactoferrin (gLf) on two cell lines, ...
Iraqi Journal of Cancer and Medical Genetics
Study The Effect of Purified Goat Milk Lactoferrin on HeLa Cancer Cell line Growth In vitro
Zainab H.Abbas1, Kifah S .Doosh2 , Nahi Y. Yaseen3 1 College of Agriculture / Karbala University 2 College of Agriculture / Baghdad University 3 Iraqi center for Caner and Medical Genetic Research / Al-Mustansiriyah Uni.
Abstract:
L
actoferrin is an important protein in many biological applications as a potential cancer treatment agent .In this study, lactoferrin was purified from goat colostrum by Ion exchange chromatography by using CM-Sephadex G-150 column and gelfiltration by using Sephadex G-200 column .To evaluted its ability as anticancer agent the study utilized an in vitro evaluation for the cytotoxic effect of the purified goat milk lactoferrin (gLf) on two cell lines, HeLa (Human cervical cancer) cell line and Rat Embryo Fibroblast (REF) cell line at different concentrations and different exposure time of treatment. The purified gLf concentrations ranging (19.53 to 5000) μg/ml for 24,48 and 72 hours. The effect of gLf was evaluated by employing MTT assay. The results revealed significant cytotoxic effect at levels (P
