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Mar 30, 2012 - Mohd A. Mohd Ridzuan1,2, Robert W. Moon1, Ellen Knuepfer1, Sally Black1, ...... Burgess DR (2005) Cytokinesis: new roles for myosin.
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Jul 20, 2007 - With N-glycan substrates, HEXO1 displayed a much higher specific activity .... cate numbers of bootstrap trials out of 1,000 that ..... substrate was converted into MM-PA or MMXF-PA, respectively. .... taxonomy.zoology.gla.ac.uk/rod/tr
Jul 10, 2010 - Brian Athey, PI) and by NIH grant U54 RR022241 (Dr. Alan Waggoner, PI). References. 1. Boland MV, Murphy RF. A neural network classifier ...
1 Center for Bioimage Informatics and Departments of Biological Sciences and Machine. Learning, Carnegie Mellon University, Pittsburgh, PA 15213.
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Asifullah Khan Ñ Abdul Majid Ñ Tae-Sun Choi. Received: 27 October 2008 / Accepted: 12 January 2009 / Published online: 7 February 2009 Ñ Springer-Verlag ...
Location Prediction. Qian Xu, Sinno Jialin Pan, Hannah Hong Xue, and Qiang Yang ...... Bureau of Nansha, Guangzhou, Guangdong, China. REFERENCES.
Q.Z. is supported by grant CA42557 to Janet Rowley. REFERENCES. 1. Baeuerle, P. A. 1991. The inducible transcription factor NF-KB: regulation by distinct ...
May 22, 2015 - oxygen (ROS)-sensitive or -responsive proteins in addition to NFkB and several ... protein, and demonstrating a differing response to selenium ...
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Oct 13, 2011 - Subcellular Heterogeneity of Ryanodine Receptor. Properties in Ventricular Myocytes with Low T-Tubule. Density. Liesbeth Biesmans1, Niall ...
The proliferative response of lymphocytes during allogeneic mixed-lymphocyte cultures is stimulated principally by antigens of a distinct region of the major.
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Published November 1, 1986
SUBCELLULAR LOCATION AND PROPERTIES OF
BACTERICIDAL FACTORS FROM HUMAN NEUTROPHILS BY
JOELLE E. GABAY,* JEANNE M. HEIPLE,t ZANVIL A. COHN,¢ CARL F. NATHAN*
AND
At least two antimicrobial systems exist in polymorphonuclear leukocytes (PMN),' one that depends on the production of reactive oxygen intermediates and another that is independent of the respiratory burst (1-3). Neutrophilderived proteins have been implicated as components of the respiratory burstindependent microbicidal pathway (4-6) . However, their nature, subcellular localization, mode of action, and actual contribution to killing in vivo are still a matter of debate . We have examined the location of bactericidal factors (BF) in human neutrophils, taking advantage of a new method for the efficient subcellular fractionation of these cells: disruption by nitrogen cavitation and centrifugation of the postnuclear supernatant on a discontinuous Percoll density gradient (7). In contrast to previous work, we have looked at the distribution of BF at each step of the fractionation procedure and in all the fractions, from whole cell lysates to isolated cellular compartments . On the basis of our localization results, we have used a highly purified organelle population, azurophilic granules, as our starting material for the isolation of azurophil-derived bactericidal factors (ADBF) . Materials and Methods Blood was obtained from healthy donors who gave informed consent . The blood was anticoagulated with 25 mM sodium citrate and mixed with an equal volume of 6% dextran in 0.9% NaCl to enhance the sedimentation of erythrocytes . After 60 min at room temperature, the leukocyte-rich supernatant was collected and centrifuged at 200 g for 10 min. The cell pellets were resuspended in 0.9% NaCl . PMN were separated from mononuclear cells by centrifugation through Ficoll-Hypaque and contaminating erythrocytes removed by two successive cycles of hypotonic lysis, as described (7, 8). >98% of the cells were PMN, of which >95% were neutrophils and
