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Supplementary Figures Figures 1-10

Control of CD8αα intestinal intraepithelial lymphocyte development by TGF-β Joanne E. Konkel, Takashi Maruyama, Andrea C. Carpenter, Yumei Xiong, Brian Zamarron, Bradford E. Hall, Ashok Kulkarni, Pin Zhang, Remy Bosselut, WanJun Chen

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a

b * Percent TCRαβ+ CD8αβ+ IEL

Percent TCRαβ+ CD8αα+ IEL

*

Tgfbr1f/+ CD45.1+ Tgfbr1f/f CD45.1+ CD4-Cre+ CD4-Cre+ WT : CD45.1+ chimeras

KO : CD45.1+ chimeras

Tgfbr1f/+ CD45.1+ Tgfbr1f/f CD45.1+ CD4-Cre+ CD4-Cre+ WT : CD45.1+ chimeras

KO : CD45.1+ chimeras

Supplementary Figure 1. Analysis of mixed bone chimeras. (a, b) IEL populations were examined in chimeric mice at 7 weeks post transfer of either CD45.1+ plus Tgfbr1f/+CD4-cre+ (WT chimeras; n=4) bone marrow or CD45.1+ plus Tgfbr1f/fCD4-cre+ (KO chimeras; n=3) bone marrow. Bone marrow was transferred at a ratio of 2:1, CD45.1 cells to CD45.2 cells. Bar graphs showing frequencies of (a) TCRab+CD8aa+ IEL and (b) TCRab+CD8αβ+ IEL in chimeric mice (mean ± SEM). *p