(Nutter Butter, Nabisco) had been buried under fresh bedding. ... The first odor (peanut butter or PB) was presented four consecutive times (PB 1, PB 2,. PB 3, PB ...
Olfactory behavior and physiology are disrupted in prion protein knockout mice Claire E. Le Pichon1, Matthew T. Va lley1, Magdalini Polymenidou2, Alexander T. Chesler1 , Botir T. Sagdullaev1 *, Adriano Aguzzi2 , and Stuart Firestein1
Supplementary information
Supplementary Figure 1 Controls for the cookie finding test. (a-b) Metabolism controls: average daily food consumption (a) and body weight (b) of B6129 (filled circles, n=9), ZI PrP–/– (empty circles, n=10), and NSE-PrP (filled grey circles, n=9) mice was similar regardless of strain. Thus, metabolic differences can be ruled out as factoring into the cookie finding phenotype of the PrP–/– mice. The animals shown here are the same B6129, PrP–/– and NSE-PrP mice as represented in Figs. 2 and 4. Black lines represent median values. (c) Visible cookie test: B6129 (filled black circles, n=10), ZI PrP–/– (empty circles, n=8), and NSE-PrP (filled grey circles, n=9) mice were subjected to a modified version of the cookie finding test where the olfactory cookie stimulus was placed on the surface of the bedding. Latencies to find the cookie were scored. Black lines denote median latencies. All mice approach the cookie within ~10 s. The PrP–/– mice are clearly able to locomote towards the cookie in a similar time range as the controls. Interestingly, the NSE-PrP are slightly faster than the other 2 strains (*p
