Suppl Figures

A

mRNA induction by serum (microarrays)

30 44,1

43,4

fold - induction

25

- OHT + OHT

20 15 10 5 0

OCR

180 B 165

C

135 120 105

OCR-RMA

ChIP-seq: OCR no serum

150 135 120 105 90 75 60 45 30 15 0

90

serum

ChIP-seq: OCR-RMA no serum serum

75 60 45 30 15 0

Perna et al. Figure S1

A

B Myc-dependent repression

Up in rat

FCS (h):

0

4 - OHT

8

0

C

4

8

+ OHT

0

4

8

- OHT 10 Induced

7.5

0

4

Super-induced without Myc

8

0

+ OHT 5

2.5

D

4 - OHT

1

0.75

8

0

4

Known Myc-repressed

8

0

+ OHT 0.5

4 - OHT

0.25

8

0

4

8

+ OHT

0.1

repressed

Perna et al. Figure S2

mRNA (fold induction)

MISR genes: 20

Smad7

Ptp4a1

Pmepa1

Snail

Ikbkb

Pcna

Lsm6

500 450

16

400

14

350

12

300

10

250

8

200

6

150

4

100

2

50

0

mRNA (fold induction)

Id3

18

048048

048048

048048

048048

048048

048048

048048

FCS (h)

5 4.5 4

048048

0

CD44

048048

Serpine1

048048

FCS (h)

NRS genes: Abl1

Bmi1

Carm1

Ino80

Stat3

Nufip2 Sec61a1 Ubb

Gtf3c2

Usp7

OHT : - (c-myc intact) + (c-myc deleted)

3.5 3 2.5 2 1.5 1 0.5 0

0808

0808

0808

0808

0808

0808

0808

0808

0808

0808

FCS (h)

Perna et al. Figure S3

A

Ccng2

Hspa1b

Psap

Armcx1

Pcdhb22

Gps2

Repression by serum (P≤ 0,05) validated by RT-PCR, but not Myc-dependency (P> 0,05) Myst4

Tcf7l2

Rgl1

Rco3

9930013L23Rik

Pltp

Trib

Fam107a

Itga3

No repression by serum Cdc40

0

4

Hdac6

8

0

Additional genes tested: mRNA (fold induction)

B

Sesn3

OHT : - (c-myc intact) + (c-myc deleted)

mRNA (fold induction)

mRNA (fold induction) mRNA (fold induction)mRNA (fold induction)

mRNA (fold induction) mRNA (fold induction)

Repression by serum (P≤ 0,05) and Myc-dependency (P≤ 0,05) validated by RT-PCR

4

Rnf10

8

0

4

8

0

4

8 FCS (h)

repressed by serum (P≤ 0,05) and Myc-dependent (P≤ 0,05)

Gadd45a

0

4

Sbsn

Cdkn2b

8

0

4

8

FCS (h)

Perna et al. Figure S4

Myc-dependent super-induction by serum (P≤ 0,05) validated by RT-PCR mRNA (fold induction)

p21

Mmp9

Fdps

Abr

mRNA (fold induction)

mRNA (fold induction)

Myc-dependent super-induction by serum (P> 0,05) not validated by RT-PCR Ccl5

Idi1

6330512M04Rik

Srxn1

Il11

Tnfaip2

Axud1

Tubb3

mRNA (fold induction)

1600029D21Rik OHT : - (c-myc intact) + (c-myc deleted)

0

4

8 FCS (h)

Perna et al. Figure S5

no serum 1.2

qChIP (% input)

1.0

y = 0.012x + 0.0361 R² = 0.72933

0.8 0.6 0.4 0.2 0.0 0

10

20

30

40

50

60

ChIPseq (OCR)

serum 4.0

qChIP (% input)

3.5

y = 0.0148x + 0.0136 R² = 0.6951

3.0 2.5 2.0 1.5 1.0 0.5 0.0 0

20

40

60

80

100

120

140

160

ChIPseq (OCR)

Perna et al. Figure S6

92000:93000

84000:85000

76000:77000

68000:69000

60000:61000

52000:53000

44000:45000

36000:37000

28000:29000

20000:21000

12000:13000

2103

4000:5000

-4000:-3000

250

-12000:-11000

-20000:-19000

-28000:-27000

-36000:-35000

-44000:-43000

-52000:-51000

-60000:-59000

-68000:-67000

-76000:-75000

-84000:-83000

-92000:-91000

-100000:-99000

Absolute number of peaks

A 1961

200

150

100

50

0

Distance from TSS

B

Perna et al. Figure S7

Perna et al. Figure S8