Supplement of Biogeosciences, 13, 2913–2926, 2016 http://www.biogeosciences.net/13/2913/2016/ doi:10.5194/bg-13-2913-2016-supplement © Author(s) 2016. CC Attribution 3.0 License.
Supplement of Temperature affects the morphology and calcification of Emiliania huxleyi strains Anaid Rosas-Navarro et al. Correspondence to: Anaid Rosas-Navarro (
[email protected]), Gerald Langer (
[email protected]), and Patrizia Ziveri (
[email protected])
The copyright of individual parts of the supplement might differ from the CC-BY 3.0 licence.
Figure 1. Sea surface temperatures in the North Pacific in February and August 2002 (http://disc.sci.gsfc.nasa.gov/giovanni/), the year in which the strain RCC1252 was collected. Regions of origin of the RCC1710 and RCC1252 strains in the Japanese coast are marked (stars).
1.8
1.8
(a)
1.8
(b)
1.6
1.4
1.2
1.2
1.2
1.0
1.0
1.0
0.8
0.8
0.8
µ
1.4
0.6
0.6
0.6
0.4
0.4
0.4
0.2
0.2
0.2
E.hux.A RCC1710 E.hux.A RCC1252 E.hux.A IAN01
0.0 -0.2 7.6
7.7
7.8
7.9
8.0
8.1
0.0
0.0
-0.2
-0.2 300
400
500 600 pCO2 [µatm]
700
800
(c)
1.6
1.4
µ
µ
1.6
80
100
120 140 2CO3 [µmol kg-1]
160
Figure 2. Growth rate in relation to the values of pH (a), pCO2 (b) and CO2− 3 concentration (c) at the end of the temperature experiment. Standard deviations of the triplicate experiment results are shown. Three different strains of E. huxleyi were used.
2
0.35
2.4
3.5
(a)
(b)
0.20
0.15
E.hux.A RCC1710 E.hux.A RCC1252 E.hux.A IAN01
Coccolith mass [pg ]
Coccolith length [µm]
Tube width [µm]
0.25
2.2
2.1
2.0
1.9
0.10 400
500 600 pCO2 [µatm]
700
800
2.5 2.0 1.5
1.0 0.5
1.8 300
(c)
3.0
2.3
0.30
0.0 300
400
500 600 pCO2 [µatm]
700
800
300
400
500 600 pCO2 [µatm]
700
800
Figure 3. Coccolith morphometry (a and b) and mass (c), in three E. huxleyi strains grown at different temperatures, in relation to the final values of pCO2 . Standard deviations of the triplicate experiment results are shown.
70
30
(a)
(b) Incomplete coccoliths [%]
Malformed coccoliths [%]
60 50 40 30 E.hux.A RCC1710 E.hux.A RCC1252 E.hux.A IAN01
20 10
25
20
15
10
5
0
0 300
400
500 600 pCO2 [µatm]
700
800
300
400
500 600 pCO2 [µatm]
700
800
Figure 4. Percentage of malformed (a) and incomplete (b) coccoliths, in three E. huxleyi strains grown at different temperatures, in relation to the final values of pCO2 . Standard deviations of the triplicate experiment results are shown.
3
