Christine Rossmann, Andreas S. Zimmermann, Christoph. Ruckenstuhl, Sabrina Büttner, Tobias Eisenberg, Wolfgang. Sattler, Ernst Malle and Frank Madeo.
Landes Bioscience
www.landesbioscience.com
Supplemental Material to: Didac Carmona-Gutiérrez, Ali Alavian-Ghavanini, Lukas Habernig, Maria Anna Bauer, Astrid Hammer, Christine Rossmann, Andreas S. Zimmermann, Christoph Ruckenstuhl, Sabrina Büttner, Tobias Eisenberg, Wolfgang Sattler, Ernst Malle and Frank Madeo The cell death protease Kex1p is essential for hypochlorite-induced apoptosis in yeast 2013; 12(11) http://dx.doi.org/10.4161/cc.24801 http://www.landesbioscience.com/journals/cc/article/24801
Supplemental Material
Supplemental Figure 1 (A-D) Representative fluorescence microscopic images of DHE (A), Annexin/PI (B), TUNEL (C) and the immunofluorescence (D) stainings of the experiments shown in Figure 2 D-G, respectively. DIC, differential interference contrast. Scale bar: 16 µm
Supplemental Figure 2 (A, B) Survival (A) and ROS production (B) of wild type and r0 cells after treatment with or without 300 µM HOCl for 16 h as determined via clonogenicity (A) and DHE→Ethidium conversion (B), respectively. Survival (A) was normalized to the untreated control. Experiments in (B) were quantified using a fluorescence plate reader. Data represent means ± s.e.m. (n = 12; ***p < 0.001). RFU, relative fluorescence unit.
