Supplemental Table 1. Primers used in qRT-PCR ...

Supplemental Table 1. Primers used in qRT-PCR experiments. Primer Name Bank1_F Bank1_R Blnk_F Blnk_R Card11_F Card11_R Cd22_F Cd22_R Cd72_F Cd72_R Cd79a_F Cd79a_R Cd79b_F Cd79b_R Ffar1_F Ffar1_R Ikzf3_F Ikzf3_R Pax5_F Pax5_R Prkcb_F Prkcb_R

Primer Sequence CACAGGCGGAACACAGACAG CTCGGAGCTTTCCCTTTCACC GCCCTCCAAGTGTTCCTCG GGCAGGCATCACATACATCTC GGAGGGCCAGCTATGGATGA GGTTGATGTAACGGCTCAGC ATGCGCGTCCATTACCTGTG TCAACGGTCCAATCATTTGCT GGACCAGAGGTTGACAAGCAC TTCCGAGGGTATGTGAGATGTAA TCTTCTTGTCATACGCCTGTTTG GATGTTAGACTGAAGGCTGAACC CGGTACCAGCAATGACAAGC TTTTTGGCTGCAAACCTCGG GCTTTCCATTGAACTTGTTAGCC CGCTGAGAGCAGCTAGGAAG CTGAATGACTACAGCTTGCCC GCTCCGGCTTCATAATGTTCT AAATTACCCGACTCCTCGGAC GAGTGGCCGTCCATTCACAA ATGAGTTCGTCACGTTCTCCT CCATACAGCAGCGATCCACAG

Citation PrimerBank: 110835718c3 PrimerBank: 110835718c3 PrimerBank: 2689189a1 PrimerBank: 2689189a1 PrimerBank: 28202025a1 PrimerBank: 28202025a1 PrimerBank: 209571539c1 PrimerBank: 209571539c1 PrimerBank: 160333100c3 PrimerBank: 160333100c3 PrimerBank: 6680892a1 PrimerBank: 6680892a1 NCBI Primer-BLAST NCBI Primer-BLAST PrimerBank: 29333354a1 PrimerBank: 29333354a1 PrimerBank: 2150044a1 PrimerBank: 2150044a1 NCBI Primer-BLAST NCBI Primer-BLAST PrimerBank: 116734871c1 PrimerBank: 116734871c1

Supplemental Table 2. RNAseq data demonstrate differential expression genes related to immune function. Provided is a panel of genes related to immune function (listed in alphabetical order), from which genes were selected for validation by qRT-PCR (Table 1). Gene Name Bank1 Blk Blnk Btk Card11 Ccl5 Ccr7 Cd19 Cd22 Cd79a Cd79b Cd86 Cr2 Cxcr5 H2-Eb2 H2-DMb2 H2-Ob Ighd Ikzf3 Il12rb1 Il1rn Il21r

Fold Change 2.73 3.07 2.07 1.82 2.20 2.39 2.63 3.30 4.39 2.78 2.70 1.62 2.39 2.60 3.41 2.62 3.21 4.24 3.46 2.01 4.27 2.43

p-value 3.2E-06 4.3E-08 4.2E-05 5.7E-04 1.3E-03 3.3E-03 1.1E-02 2.9E-06 1.3E-05 2.4E-04 1.1E-04 8.0E-04 3.0E-02 3.7E-06 9.0E-03 8.0E-05 5.5E-05 3.8E-05 3.2E-06 2.8E-02 6.4E-05 9.4E-03

FDR 8.2E-04 2.3E-05 4.7E-03 2.9E-02 4.7E-02 8.9E-02 1.9E-01 7.8E-04 2.1E-03 1.6E-02 8.7E-03 3.5E-02 3.4E-01 8.7E-04 1.7E-01 7.3E-03 5.5E-03 4.4E-03 8.2E-04 3.3E-01 6.2E-03 1.7E-01

Ensembl Gene ID ENSMUSG00000037922 ENSMUSG00000014453 ENSMUSG00000061132 ENSMUSG00000031264 ENSMUSG00000036526 ENSMUSG00000035042 ENSMUSG00000037944 ENSMUSG00000030724 ENSMUSG00000030577 ENSMUSG00000003379 ENSMUSG00000040592 ENSMUSG00000022901 ENSMUSG00000026616 ENSMUSG00000047880 ENSMUSG00000067341 ENSMUSG00000037548 ENSMUSG00000041538 ENSMUSG00000104213 ENSMUSG00000018168 ENSMUSG00000000791 ENSMUSG00000026981 ENSMUSG00000030745

Gene Name Lepr Ltb Malt1 Pax5 Pik3ap1 Plcg2 Pou2f2 Prkcb Ptpn6 Slamf1 Spp1 Syk Tec Tlr1 Tnfrsf13b Tnfrsf13c Trem2

Fold Change 0.45 2.02 1.60 3.66 1.69 1.71 2.01 2.04 1.79 2.41 3.02 1.93 1.54 2.07 2.08 3.46 3.26

p-value 8.2E-04 4.9E-02 2.6E-04 5.3E-06 6.5E-05 1.3E-03 4.6E-05 6.9E-06 1.4E-03 3.6E-02 6.8E-04 6.9E-05 1.3E-03 9.0E-04 1.2E-03 2.2E-03 1.4E-08

FDR 3.5E-02 4.5E-01 1.7E-02 1.2E-03 6.2E-03 4.6E-02 4.9E-03 1.3E-03 4.7E-02 3.8E-01 3.1E-02 6.5E-03 4.7E-02 3.7E-02 4.5E-02 6.7E-02 8.9E-06

Ensembl Gene ID ENSMUSG00000057722 ENSMUSG00000024399 ENSMUSG00000032688 ENSMUSG00000014030 ENSMUSG00000025017 ENSMUSG00000034330 ENSMUSG00000008496 ENSMUSG00000052889 ENSMUSG00000004266 ENSMUSG00000015316 ENSMUSG00000029304 ENSMUSG00000021457 ENSMUSG00000029217 ENSMUSG00000044827 ENSMUSG00000010142 ENSMUSG00000068105 ENSMUSG00000023992

Supplemental Figure 1. Evaluation of abdominal and organ-specific ID8 tumor burden in situ of young (Y) and aged (A) nulliparous C57Bl/6 mice. Virgin mice were injected IP with 2.52x106 ID8 mouse ovarian cancer cells tagged with RFP. Mice were sacrificed at 8 weeks post IP injection and each abdominal cavity was exposed and imaged. Tumor area and intensity were quantified as described in Materials and Methods. Abdominal Tumor Area and Abdominal Tumor Intensity were calculated by dividing either the tumor area or the tumor intensity by the scale-adjusted body weight of each mouse. P-values for tumor area and tumor intensity are 0.1677 and 0.2010, respectively. Individual organs were dissected from the mouse peritoneal cavity and imaged ex vivo. The Organ Area Fraction and Organ Intensity Fraction were calculated by dividing the tumor area or tumor intensity by the adjusted organ weight. P-values for Organ Area Fraction are as follows: FatL: 0.05; FatR: 0.03. P-values for Organ Intensity Fraction are as follows: FatL: 0.04, FatR: 0.03. Double asterisk indicates significant p-value