Supplementary Figure 4 - Nature

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native Sf3b1 mRNA. 1. 3. 2. 2. 1. 3. 1. 1. 2. 1. 2. 1. 2. Clone C8. Clone D9. Clone D9 post-FLP & post-Cre. Clone D11. NIH-3T3. W. T ES cells. W. T ES cells. W.
Water

WT ES cells

NIH-3T3

Water

WT ES cells

NIH-3T3

Clone D11

Clone D9

Clone C8

Water

WT ES cells

Clone D11

Clone D9

b

RT- PCR

Clone C8

a

native Sf3b1 mRNA 1

2

Chimeric Sf3b1 mRNA

1

3

1

3

1

1

Post-Flpe > post-Cre 6

7

8 9

st -C re 14 16 12 13 15 17

18

19

10 11

12 13

18

19

15

17

ls

ce l W at er

10 11

T

8 9

W

7

Cl

6

on

e

Native

ES

D9

po

st -F

LP

&

po

1 16_19_CO_F 2 2054_CO_R 3 16_19_CO_R

1 1357_F 2 2020_R

2

cDNA

2

c

2

genomic DNA

d

pre-Cre

post-Cre

cDNA

Supplementary Figure 4