Supplementary Information 12 Supplementary figures with legends and 5 Supplementary tables
Ubiquitin C-terminal hydrolase37 regulates Tcf7 DNA binding for the activation of Wnt signalling
Wonhee Han1, Hyeyoon Lee1 and Jin-Kwan Han1*
1
Department of Life Sciences, Pohang University of Science and Technology, 77
Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 37673, Korea
*
Author for correspondence (
[email protected])
Figure S1 Human Mouse
X.laevis Human Mouse
X.laevis Human Mouse
X.laevis Human Mouse
X.laevis Human Mouse
X.laevis Human Mouse
X.laevis
St.38
St.28
St.20
St.18
St.12
St.8
(-)
egg
a
St.10.5
Figure S2
Uch37 ODC
b
Lateral
Stage 11
Stage 10.5
Stage 9
4 cell
Animal
c (-) Uch37 Chd Msx1 Xbra ODC
WE
AC
DMZ VMZ
Vegetal
Figure S3
b +
-
-
-
Uch37 MO
-
-
+
+
+
Uch37-HA
-
+
+
-
-
Re.Uch37-HA
-
-
-
+
-
Human Uch37-HA
-
-
-
-
+
VMZ
DMZ
Mild
-
Severe
Co MO
Normal
a
HA Actin
c
d DMZ
100
100
80
80
60
Normal
40
Mild Severe
20
% of embryos
% of embryos
VMZ
Normal
60
Mild
40
Severe
20 0
0 Co MO
Uch37 MO Uch37 MO + Re.Uch37
Co MO
Uch37 MO Uch37 MO + Re.Uch37
Figure S4
a
Co MO (-)
WE
Ctl
Co MO
Uch37 MO
Wnt8
b
Wnt8
siamois
Sizzled
ODC
ODC
Co MO
c WE
(-)
Ctl
Uch37 MO
Xnr1
ODC
Fold induction
f sh Uch37
sh Control Actin
3
2
eFGF
Ctl
p
