Supplementary Information - Nature

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Lef1. Co MO. Uch37 MO. Figure S8. Uch37 IN. -. -. -. -. +. Uch37 WT ... Lef1 + Uch37 MO. Tcf7 + Co MO .... (e) Short hairpin RNA (shRNA) of Uch37 efficiently ...
Supplementary Information 12 Supplementary figures with legends and 5 Supplementary tables

Ubiquitin C-terminal hydrolase37 regulates Tcf7 DNA binding for the activation of Wnt signalling

Wonhee Han1, Hyeyoon Lee1 and Jin-Kwan Han1*

1

Department of Life Sciences, Pohang University of Science and Technology, 77

Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 37673, Korea

*

Author for correspondence ([email protected])

Figure S1 Human Mouse

X.laevis Human Mouse

X.laevis Human Mouse

X.laevis Human Mouse

X.laevis Human Mouse

X.laevis Human Mouse

X.laevis

St.38

St.28

St.20

St.18

St.12

St.8

(-)

egg

a

St.10.5

Figure S2

Uch37 ODC

b

Lateral

Stage 11

Stage 10.5

Stage 9

4 cell

Animal

c (-) Uch37 Chd Msx1 Xbra ODC

WE

AC

DMZ VMZ

Vegetal

Figure S3

b +

-

-

-

Uch37 MO

-

-

+

+

+

Uch37-HA

-

+

+

-

-

Re.Uch37-HA

-

-

-

+

-

Human Uch37-HA

-

-

-

-

+

VMZ

DMZ

Mild

-

Severe

Co MO

Normal

a

HA Actin

c

d DMZ

100

100

80

80

60

Normal

40

Mild Severe

20

% of embryos

% of embryos

VMZ

Normal

60

Mild

40

Severe

20 0

0 Co MO

Uch37 MO Uch37 MO + Re.Uch37

Co MO

Uch37 MO Uch37 MO + Re.Uch37

Figure S4

a

Co MO (-)

WE

Ctl

Co MO

Uch37 MO

Wnt8

b

Wnt8

siamois

Sizzled

ODC

ODC

Co MO

c WE

(-)

Ctl

Uch37 MO

Xnr1

ODC

Fold induction

f sh Uch37

sh Control Actin

3

2

eFGF

Ctl

p