Supplementary information, Table S1 Data collection and ... - Nature

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Refmac & Phenix. Average Fourier shell correlation. 0.84. 0.77. 0.85. 0.90. R-factor. 0.30. 0.30. 0.30. 0.28. Validation. R.m.s deviations. Bonds length (Å). 0.01.
Supplementary information, Table S1 Data collection and model statistics Data collection EM equipment Voltage (kV) Detector

FEI Tecnai Polara 300 Falcon III

Pixel size (Å) Electron dose (e-/Å2)

1.34 40 1.8-5

Defocus range (m) Micrographs Reconstruction Software

RELION 1.4

Maps

C1

C3

Symmetry

C4

open

C4

Final Resolution (Å) (Å2)

Map sharpening B-factor Accuracy of rotations (o) Accuracy of translation (pixels)

3.8 110

4.2 110

4.0 130

5.7 450

0.7 0.6

0.7 0.6

0.8 0.7

1 0.9

0.84

Refmac & Phenix 0.77 0.85

0.90

0.30

0.30

0.30

0.28

0.01 1.32

0.01 1.56

0.01 1.47

0.01 1.58

92.2 5.5 2.3

91.6 6.1 2.3

91.0 6.1 2.9

90.5

Model building Software Refinement Software Average Fourier shell correlation R-factor Validation R.m.s deviations Bonds length (Å) Bonds Angle (o) Ramachandran plot statistics (%) Preferred Allowed Outlier

DireX & Coot

6.8 2.7