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Supplementary information, Table S1 Data collection and ... - Nature
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Supplementary information, Table S1 Data collection and ... - Nature
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Refmac &
Phenix
. Average Fourier shell correlation. 0.84. 0.77. 0.85. 0.90. R-factor. 0.30. 0.30. 0.30. 0.28. Validation. R.m.s deviations. Bonds length (Ã ). 0.01.
Supplementary information, Table S1 Data collection and model statistics Data collection EM equipment Voltage (kV) Detector
FEI Tecnai Polara 300 Falcon III
Pixel size (Å) Electron dose (e-/Å2)
1.34 40 1.8-5
Defocus range (m) Micrographs Reconstruction Software
RELION 1.4
Maps
C1
C3
Symmetry
C4
open
C4
Final Resolution (Å) (Å2)
Map sharpening B-factor Accuracy of rotations (o) Accuracy of translation (pixels)
3.8 110
4.2 110
4.0 130
5.7 450
0.7 0.6
0.7 0.6
0.8 0.7
1 0.9
0.84
Refmac & Phenix 0.77 0.85
0.90
0.30
0.30
0.30
0.28
0.01 1.32
0.01 1.56
0.01 1.47
0.01 1.58
92.2 5.5 2.3
91.6 6.1 2.3
91.0 6.1 2.9
90.5
Model building Software Refinement Software Average Fourier shell correlation R-factor Validation R.m.s deviations Bonds length (Å) Bonds Angle (o) Ramachandran plot statistics (%) Preferred Allowed Outlier
DireX & Coot
6.8 2.7
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