Supplementary Material (ESI) - Royal Society of Chemistry

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Slope Y-intercept r2. Nominal conc. (nM). 50 100 200 300 400 500. Measured conc. (nM) 49 102 201 305 407 489. Recovery (%). 98 102 99 102 102 102. 1.24.
Supplementary Material (ESI) for Journal of Analytical Atomic Spectrometry This journal is © The Royal Society of Chemistry 2008 Electronic Supplementary Data Table S1. Data on calibration curve. a Linear regression parameters

Calibration standard curve

a

Nominal conc. (nM)

50

100

200

300

400

500

Measured conc. (nM)

49

102

201

305

407

489

Recovery (%)

98

102

99

102

102

102

Slope

Y-intercept

r2

1.24

2.10

0.9974

All measured data values are expressed as mean of 3 repeats with coefficient of

variation of ≤ 10%

Table S2. Intra- and inter-assay recovery and precision. a Oxaliplatin conc. (nM) % Recovery Precision (%C.V.) Intra-assay

Inter-assay

a

50

101

7.12

200

107

4.35

500

106

5.22

50

102

3.30

200

104

5.65

500

103

4.39

All measured data values are expressed as mean of ≥ 5 repeats with coefficient of

variation of ≤ 10%.

Supplementary Material (ESI) for Journal of Analytical Atomic Spectrometry This journal is © The Royal Society of Chemistry 2008 Table S3. Stability of oxaliplatin in MDP under different conditions. All data values are expressed as mean ± standard deviation of 3 repeats. Recovery (%) of oxaliplatin after indicated time On Ice

Time (h)

At -80 °C

2

4

109 ± 7

112 ± 3.7

1

4

9

90

104 ± 4.1

110 ± 6.9

111 ± 4.6

98 ± 1.3

Time (day)

Fig. S1. HPLC-ICP-MS chromatogram of stability samples of MDP containing oxaliplatin stored on ice for 4 h (A) and at -80ºC for 90 days (B).

3.2

A

2.4

1.6

8

0

0

B

OP

Abundance (cps×10 4 )

Abundance (cps×10 4 )

3.2

5

10

15

Time (min)

20

OP

2.4

1.6

8

0 0

5

10

Time (min)

15

20

Supplementary Material (ESI) for Journal of Analytical Atomic Spectrometry This journal is © The Royal Society of Chemistry 2008 Fig. S2. Analysis of plasma from colorectal cancer patients showing concentrations of oxaliplatin during and after a 2-h infusion (A), and a chromatogram of 1-h postinfusion sample (B). 6.0

B

A

8

A b u n d a n c e (c p s × 1 0 3 )

C o n cen tr atio n (µ M )

10

6 4 2

4.5 OP 3.0

1.5

2-h infusion

0 0

1

2

Time (hours)

3

4

0 0

5

10

Time (min)

15

20