Supplementary Material Presence of azaspiracids in bivalve molluscs from Northern Spain Juan Blanco1, Fabiola Arévalo2, Ángeles Moroño2; Jorge Correa2, Susana Muñíz2, Carmen Mariño1, Helena Martín1 1
CIMA (Centro de Investigacións Mariñas), Pedras de Corón s/n, Vilanova de Arousa, 36620, Spain 2
Intecmar (Instituto Tecnolóxico para o Control do Medio Mariño de Galicia), Peirao de Vilaxoán s/n, Vilagarcía de Arousa, 36611, Spain
1
Corresponding author. E-mail
[email protected]; phone +34 886206340; E-mail Adresses: F. Arévalo:
[email protected]; A. Moroño:
[email protected]; J. Correa:
[email protected]; C. Mariño:
[email protected]; H. Martín:
[email protected]
Sampling areas for cultured mussel (rafts) in Galicia (http://sig.intecmar.gal/EstadoZonas/Default.aspx?tmapa=0)
Figure 1S. Sampling areas for cultured mussels in the Ría de Muros
Figure 2S. Sampling areas for cultured mussels in the Ría de Arousa
Figure 3S. Sampling areas for cultured mussels in the Rías de Pontevedra (upper) and Vigo
Figure 4S. Sampling areas for cultured mussels in the Ría de Sada
Sampling areas for infaunal bivalves in Galicia (http://sig.intecmar.gal/EstadoZonas/Default.aspx?tmapa=1)
Figure 5S. Sampling areas for infaunal bivalves from the Ría de Vigo to Fisterra
Figure 6S. Sampling areas for infaunal bivalves from Fisterra to Cedeira.
Figure 7S. Sampling areas for infaunal bivalves from Cedeira to Ribadeo.
Table IS. Samples selected for concentration and confirmation. B = Basque Country, C = Cantabria, A = Asturias, GC = Galician Coast, RM = Ría de Muros, RA = Ría de Arousa, RP= Ría de Pontevedra, RV = Ría de Vigo Date Species 07/06/2016 Mytilus galloprovincialis (2) 15/11/2016 Mytilus galloprovincialis (1) 15/11/2016 Ensis siliqua (2) 23/11/2016 Mytilus galloprovincialis (1) 07/12/2016 Ruditapes romboides (2) 20/12/2016 Cerastoderma edule (2) 26/12/2016 Mytilus galloprovincialis (1) 10/01/2017 Cerastoderma edule (2) 09/01/2017 Crassostrea gigas (2) 16/01/2017 Mytilus galloprovincialis (1) 07/02/2017 Mytilus galloprovincialis (2) 07/02/2017 Venerupis pullastra (2) (1) Suspended cultures (2) Natural beds
Location Mutriku Muros-B Fisterra Grove-C Vigo-I Corcubión Grove-C Barqueiro Mundaka Cangas-B Anllóns Vigo IV
Area B RM GC RA RV GC RA GC B RP GC RV
Sample number 1018 5769 5788 5998 6347 6613 6742 138 169 207 471 473
Table IIS. Number of peaks, corresponding to the transitions (two measured) of the AZAs in the header, detected in concentrated samples along two time-ranges (2.5-3.3 and 3.3-5 min) of the chromatographic run. + = trace, ? = unclear. min 2.5-3.3
min 3.3-5
Sample number 138
3 -
43 1
4 1
5 -
1 2
6 -
3 -
43 37 2 - 1 2
169 207 471 473 1018
1 1 -
1 1 -
-
-
2 2 2 2 -
2 2 2
1 2 -
? -
1 1 1 1 1
5769 5788
2 1
2 1
1
1
2 2
2 -
-
2 -
1 1
12 +, 2 +?? 2 1,+ 2 1,+ 1? 2 1,+? 1? 2 +, 2 +,+? +?? 2 1,+? -
5998 6347 6613 6742
1,+ 1 -
1,+ 1 -
1 1 -
1 -
2 2 2 2
2 2
1? -
-
1 1 1 1
2 +,+? 2 2 2 2 2 2 2 1,+?
11 1,+
Figure 8S. Chromatograms of the sample number 473 corresponding to the confirmation transitions between min 2.5 and 3.3.
Figure 9S. Chromatograms of the sample number 473 corresponding to the confirmation transitions between min 3.3 and 5 (part 1).
Figure 10S. Chromatograms of the sample number 473 corresponding to the confirmation transitions between min 3.3 and 5 (part 2).
Figure 11S. Chromatograms of the sample number 471 corresponding to the confirmation transitions between min 2.5 and 3.3.
Figure 12S. Chromatograms of the sample number 471 corresponding to the confirmation transitions between min 3.3 and 5 (part 1).
Figure 13S. Chromatograms of the sample number 471 corresponding to the confirmation transitions between min 3.3 and 5 (part 2).
Figure 14S. Chromatograms of the sample number 207 corresponding to the confirmation transitions between min 2.5 and 3.3.
Figure 15S. Chromatograms of the sample number 207 corresponding to the confirmation transitions between min 3.3 and 5 (part 1).
Figure 16S. Chromatograms of the sample number 207 corresponding to the confirmation transitions between min 3.3 and 5 (part 2).
Figure 17S. Chromatograms of the sample number 138 corresponding to the confirmation transitions between min 2.5 and 3.3.
Figure 18S. Chromatograms of the sample number 138 corresponding to the confirmation transitions between min 3.3 and 5 (part 1).
Figure1 9S. Chromatograms of the sample number 138 corresponding to the confirmation transitions between min 3.3 and 5 (part 2).
Figure 20S. Chromatograms of the sample number 6742 corresponding to the confirmation transitions between min 2.5 and 3.3.
Figure 21S. Chromatograms of the sample number 6742 corresponding to the confirmation transitions between min 3.3 and 5 (part 1).
Figure 22S. Chromatograms of the sample number 6742 corresponding to the confirmation transitions between min 3.3 and 5 (part 2).
Figure 23S. Chromatograms of the sample number 6613 corresponding to the confirmation transitions between min 2.5 and 3.3.
Figure 24S. Chromatograms of the sample number 6613 corresponding to the confirmation transitions between min 3.3 and 5 (part 1).
Figure 25S. Chromatograms of the sample number 6613 corresponding to the confirmation transitions between min 3.3 and 5 (part 2).
Figure 26S. Chromatograms of the sample number 1018 corresponding to the confirmation transitions between min 2.5 and 3.3.
Figure 27S. Chromatograms of the sample number 1018 corresponding to the confirmation transitions between min 3.3 and 5 (part 1).
Figure 28S. Chromatograms of the sample number 1018 corresponding to the confirmation transitions between min 3.3 and 5 (part 2).
Figure 29S. Chromatograms of the sample number 5788 corresponding to the confirmation transitions between min 2.5 and 3.3.
Figure 30S. Chromatograms of the sample number 5788 corresponding to the confirmation transitions between min 3.3 and 5 (part 1).
Figure 31S. Chromatograms of the sample number 5788 corresponding to the confirmation transitions between min 3.3 and 5 (part 2).
Figure 32S. Chromatograms of the sample number 5998 corresponding to the confirmation transitions between min 2.5 and 3.3.
Figure 33S. Chromatograms of the sample number 5998 corresponding to the confirmation transitions between min 3.3 and 5 (part 1).
Figure 34S. Chromatograms of the sample number 5998 corresponding to the confirmation transitions between min 3.3 and 5 (part 2).
Figure 35S. Chromatograms of the sample number 169 corresponding to the confirmation transitions between min 2.5 and 3.3.
Figure 36S. Chromatograms of the sample number 169 corresponding to the confirmation transitions between min 3.3 and 5 (part 1).
Figure 37S. Chromatograms of the sample number 169 corresponding to the confirmation transitions between min 3.3 and 5 (part 2).
Figure 38S. Chromatograms of the sample number 6347 corresponding to the confirmation transitions between min 2.5 and 3.3.
Figure 39S. Chromatograms of the sample number 6347 corresponding to the confirmation transitions between min 3.3 and 5 (part 1).
Figure 40S. Chromatograms of the sample number 6347 corresponding to the confirmation transitions between min 3.3 and 5 (part 2).
Figure 41S. Chromatograms of the sample number 5769 corresponding to the confirmation transitions between min 2.5 and 3.3.
Figure 42S. Chromatograms of the sample number 5769 corresponding to the confirmation transitions between min 3.3 and 5 (part 1).
Figure 43S. Chromatograms of the sample number 5769 corresponding to the confirmation transitions between min 3.3 and 5 (part 2).
