Supplementary Table 1: Antibodies used to detect

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BD Bioscience. BV786. Cryopreserved PBMC. 4. S3.5. Invitrogen. Q-dot 605. 1. Cryopreserved whole blood ... (Ref L34957). Thermo-Fisher. Scientific. Aqua.
Supplementary Table 1: Antibodies used to detect memory markers, chemokine receptors, cytotoxic molecules and cytokines expression profiles of CD4+ T cells.

CCR7 CXCR3 CD3

CD4

CD45RA

CD27

Manufacturer BD Biosciences BD Biosciences BD Biosciences

150503

BD Biosciences

150503 1C6/CXCR3 UCHT1 MOPC-21 L200 SK3

eBioscience BD Biosciences BD Biosciences BD Biosciences BioLegend BD Bioscience

Flourochrome BV421 BV711 BV605 BV711 PerCP Cy5.5 PE-CF594 PE PE-Cy 5 Alexa Fluor 700 BV 431 BV785 BV786

S3.5

Invitrogen

Q-dot 605

HI100 HI100 HI100 HI100 L128 M-T271

BD Biosciences eBioscience BioLegend eBioscience

APC H7

BD Biosciences Biolegend

CD95

DX2 L243 SK1 RPA-T8 M5E2 HIB19 RPA-T8 M5E2 SJ25C1 CB9 GB11

Granzyme K

GM6C3

Perforin IL-2 IL-17 IFNTNF-

G9 5344.111 SCPL1362 B27 Mb11

Live/Dead

N/A

Live/Dead

(Ref L34957)

R & D Systems BD Biosciences BD Biosciences eBioscience BD Biosciences eBioscience Thermo-Fisher Scientific Thermo-Fisher Scientific

Cryopreserved PBMC Cryopreserved PBMC Fresh/Cryopreserved PBMC Cryopreserved whole blood Fresh PBMC Fresh/Cryopreserved PBMC Cryopreserved whole blood Fresh/Cryopreserved PBMC Cryopreserved PBMC Cryopreserved whole blood Cryopreserved PBMC Fresh/Cryopreserved PBMC Cryopreserved whole blood Fresh/Cryopreserved PBMC Cryopreserved PBMC Fresh/Cryopreserved PBMC Cryopreserved PBMC Cryopreserved whole blood

BV421

Cryopreserved PBMC

BV510

Fresh/Cryopreserved PBMC

Intracellular Surface Intracellular Surface Intracellular Surface Intracellular

Intracellular

Panel 3 4 1 2-3 5 3 1-4 5 2-3 4 1 5 4 1 2-3 5 2-4 1 4 2-3 2 5 1

2-4

PE-Cy 7 Pac Blue FITC PE-Cy 5 (in-house conjugation) PE-CF594 FITC Alexa Fluor 647 Alexa Fluor 700 PE-Cy 7

Cryopreserved PBMC

Intracellular

Granulysin

PolyClonal (Ref AF3138)

BioLegend BD Biosciences BD Biosciences BioLegend BD Biosciences BioLegend BioLegend BD Biosciences eBioscience BioLegend Santa Cruz Biotechnology

BV570 BV650 FITC APC BV605 FITC BV421 PerCP-Cy5.5

Fresh PBMC

Staining

Surface

HLA-DR CD8 CD8 CD14 CD19 CD8 CD14 CD19 Granzyme A Granzyme B

BD Biosciences

PE-Cy 7

Sample Type

Surface

Clone 1G1 2D7 11A9

Surface

Marker CCR4 CCR5 CCR6

Cryopreserved whole blood

4

5

ViViD

Cryopreserved PBMC

Surface

1

Aqua

Fresh/Cryopreserved PBMC

Surface

2-4