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Supplementary Table 1: Antibodies used to detect
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Supplementary Table 1: Antibodies used to detect
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BD Bioscience. BV786. Cryopreserved PBMC. 4. S3.5.
Invitrogen
. Q-dot 605. 1. Cryopreserved whole blood ... (Ref
L34957
). Thermo-Fisher. Scientific. Aqua.
Supplementary Table 1: Antibodies used to detect memory markers, chemokine receptors, cytotoxic molecules and cytokines expression profiles of CD4+ T cells.
CCR7 CXCR3 CD3
CD4
CD45RA
CD27
Manufacturer BD Biosciences BD Biosciences BD Biosciences
150503
BD Biosciences
150503 1C6/CXCR3 UCHT1 MOPC-21 L200 SK3
eBioscience BD Biosciences BD Biosciences BD Biosciences BioLegend BD Bioscience
Flourochrome BV421 BV711 BV605 BV711 PerCP Cy5.5 PE-CF594 PE PE-Cy 5 Alexa Fluor 700 BV 431 BV785 BV786
S3.5
Invitrogen
Q-dot 605
HI100 HI100 HI100 HI100 L128 M-T271
BD Biosciences eBioscience BioLegend eBioscience
APC H7
BD Biosciences Biolegend
CD95
DX2 L243 SK1 RPA-T8 M5E2 HIB19 RPA-T8 M5E2 SJ25C1 CB9 GB11
Granzyme K
GM6C3
Perforin IL-2 IL-17 IFNTNF-
G9 5344.111 SCPL1362 B27 Mb11
Live/Dead
N/A
Live/Dead
(Ref L34957)
R & D Systems BD Biosciences BD Biosciences eBioscience BD Biosciences eBioscience Thermo-Fisher Scientific Thermo-Fisher Scientific
Cryopreserved PBMC Cryopreserved PBMC Fresh/Cryopreserved PBMC Cryopreserved whole blood Fresh PBMC Fresh/Cryopreserved PBMC Cryopreserved whole blood Fresh/Cryopreserved PBMC Cryopreserved PBMC Cryopreserved whole blood Cryopreserved PBMC Fresh/Cryopreserved PBMC Cryopreserved whole blood Fresh/Cryopreserved PBMC Cryopreserved PBMC Fresh/Cryopreserved PBMC Cryopreserved PBMC Cryopreserved whole blood
BV421
Cryopreserved PBMC
BV510
Fresh/Cryopreserved PBMC
Intracellular Surface Intracellular Surface Intracellular Surface Intracellular
Intracellular
Panel 3 4 1 2-3 5 3 1-4 5 2-3 4 1 5 4 1 2-3 5 2-4 1 4 2-3 2 5 1
2-4
PE-Cy 7 Pac Blue FITC PE-Cy 5 (in-house conjugation) PE-CF594 FITC Alexa Fluor 647 Alexa Fluor 700 PE-Cy 7
Cryopreserved PBMC
Intracellular
Granulysin
PolyClonal (Ref AF3138)
BioLegend BD Biosciences BD Biosciences BioLegend BD Biosciences BioLegend BioLegend BD Biosciences eBioscience BioLegend Santa Cruz Biotechnology
BV570 BV650 FITC APC BV605 FITC BV421 PerCP-Cy5.5
Fresh PBMC
Staining
Surface
HLA-DR CD8 CD8 CD14 CD19 CD8 CD14 CD19 Granzyme A Granzyme B
BD Biosciences
PE-Cy 7
Sample Type
Surface
Clone 1G1 2D7 11A9
Surface
Marker CCR4 CCR5 CCR6
Cryopreserved whole blood
4
5
ViViD
Cryopreserved PBMC
Surface
1
Aqua
Fresh/Cryopreserved PBMC
Surface
2-4
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