Supplementary Table 1. List of primer pairs used in ...

1

Kl_Komy et al.

Supplementary Table 1. List of primer pairs used in this study Primer Target pair pathogena

Target protein (Locus id)

Forward primer (5’-3’)

Reverse primer (5’-3’)

Ta (°C)b

Expected product (bp)

Hypothetical protein CTTAAGGGTGTTGTGGCAGCAG ACTTCGCGAGTAGACCCTCAAC 55 682 (CMN_01176) Hypothetical protein Cmn-2 Cmn CTTGGGTACGCAACGAATCCAC AACCATCGCTCATCGTCTGTCA 53.4 537 (CMN_02187) Hypothetical protein Cmn-3 Cmn GTGATCATCAACATCGCGGTCG GCAGCGGATGCGTTCTATGG 54.5 567 (CMN_00235) Hypothetical protein Cmn-4 Cmn GCCCGCCGCTTCTATTTACATC TGACGACAATACGCTTCCCCTT 53.4 864 (CMN_02866) Hypothetical protein Cmn-5 Cmn CACTTTGGAGCAGCGTCAAGAG GAACCCGCGACTTCCTCTTTTG 55 720 (CMN_01921) Hypothetical protein Pss2-1 Pss TGCTGTCGTTACTTCACACGGA AATGTGCCAGCTGCCAGTAGTA 53.4 307 (CKS_5651) Hypothetical protein Pss2-2 Pss ACAACTATGTACGCGAACCGGA TATCAACGGCATTTGCAGCCTG 53.4 483 (CKS_1982) Relaxase Pss2-3 Pss CGGTGATTTCAGGGTCATTGGC CAGCAGCCCGGTATGGATATCA 55 424 (CKS_5544) Antiterminater Q Pss2-4 Pss GCGGTAAATAAGCTGCCCGATC CCACATGCGCAATTACCTCGTT 53.4 364 (CKS_5565) Hypothetical protein Pss2-5 Pss GGGATGATGATGGCGATCAGGA CCATCCACACCATGAGTCTGGT 55 332 (CKS_1899) Hypothetical protein Pss2-7 Pss TTCGTTGATAATTTCGGCCCGC AGTTCGGCGTAGTAGGCTTTGA 53.4 359 (CKS_5607) Hypothetical protein Pss2-9 Pss TGGTCGCTATGGGCCAGTTTAA TTACCGTGTGACATATCCCGGG 53.4 337 (CKS_2975) Pss2Hypothetical protein Pss GGTGTTTCTGAACCGCACACAA ATTTTTAGCCAGTTCAGCCCGC 53.4 448 10 (CKS_5633) Hypothetical protein Rt-1 Rt TTGACCATCTGGAAGTCGACCC ACCGACCCGACAAAGGACATC 60 578 (A6122_2884) CAAX protease Rt-2 Rt ATCAGTTCTACCCTGGCACCAC TGAGAGATCGGAACAAGCTGCA 60 673 (A6122_2873) Hypothetical protein Rt-3 Rt GTGGGCTGATAGGTGGTGATGT GCGCCCTTTCTCTACTGGGTAT 60 520 (A6122_2866) Hypothetical protein Rt-4 Rt AAGCGGAGACCTCATTGTCGAA GCTTCGATTGTCACTTTCGCCA 60 580 (A6122_2803) Hypothetical protein Rt-5 Rt GAGCCACTCTTCCATGACACCT GCTTGTCGCAGAAAAAGGCTCT 60 613 (A6122_2790) a Cmn, Clavibacter michiganensis subsp. nebraskensis; Pss, Pantoea stewartii subsp. stewartii; Rt, Rathayibacter tritici, bannealing temperature Cmn-1

Cmn

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Kl_Komy et al.

Supplementary Fig. 1. Sensitivity of Cmn primers estimated by real-time PCR with primer pairs Cmn-3 (A) and Cmn-4 (B). The linear regression generated by ten-fold dilution of DNA of Clavibacter michiganensis subsp. nebrskensis NCPPB 2581 and converted into copy number of the target gene corresponding the number of bacterial cells.

Supplementary Fig. 2. Sensitivity of Pss primers estimated by real-time PCR with primer pairs Pss2-4 (A) and Pss2-9 (B). The linear regression generated by ten-fold dilution of DNA of Pantoea stewartii subsp. stewartii NCPPB 2295 and converted into copy number of the target gene corresponding the number of bacterial cells.

Supplementary Fig. 3. Sensitivity of Rt primers estimated by real-time PCR with primer pairs Rt-4 (A) and Rt-5 (B). The linear regression generated by ten-fold dilution of DNA of Rathayibacter tritici NCPPB 1857 and converted into copy number of the target gene corresponding the number of bacterial cells.