Supporting Information
Efficient production of tri-acetylated mono-acylated mannosylerythritol lipids by Sporisorium sp. aff. sorghi SAM20
Nayyereh Alimadadi1, Mohammad Reza Soudi1, Zahra Talebpour2
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Department of Microbiology, Faculty of Biological Sciences, Alzahra University,
Tehran, Iran 2
Department of Chemistry, Faculty of Physics & Chemistry, Alzahra University,
Tehran, Iran
Correspondence Mohammad Reza Soudi, Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran. E-mail:
[email protected] Tel: +98-21-88044058-9 (2441); Fax: +98-21-88058912
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Table S1 Analysis of soybean oil Test Iodine value (% of iodine adsorbed) * Saponification value (mg KOH g−1) * Peroxide value (mmol kg−1) * Fatty acid composition (%) †: C16:0 C18:0 C18:1 C18:2 Others
Value 114 145 2.5 8.3 6.5 32.0 50.9 2.3
*
The tests were performed following the A.O.C.S. official methods of analysis (Firestone, 1994). Fatty acid composition of soybean oil was determined using the same procedure applied for fatty acid profiling of MELs which mentioned in the article. †
Table S2 Similarity between the sequences of the LSU rDNA D1/D2 domains and ITS region of Sporisorium sp. aff. sorghi SAM20 and the closely related species Closely related species D1/D2 ITS (Accession Numbers: D1/D2; ITS) Identity Nucleotide Identity Nucleotide (%) difference* (%) difference Sporisorium sorghi CBS 104.17 99.7 2 (2 gaps)/605 99.4 3 (1 gap)/661 (AY745726/DQ200931) Sporisorium cruentum MS14 99.7 2 (2 gaps)/605 99.4 4 (2 gaps)/729 (AY740156) Sporisorium foveolati MS21 99.7 2 (2 gaps)/605 98.0 14 (10 gaps)/685 (AY740103/AY740050) Sporisorium moniliferum MS98 99.7 2 (2 gaps)/605 97.9 15 (9 gaps)/720 (AF453940/AY344984) * Number of gaps is included in the nucleotide differences.
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Figure S1 Colonies (a) and cells (b) of Sporisorium sp. aff. sorghi SAM20 grown on 5% malt extract agar for 5 days at 17 °C
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Figure S2 Partial 1H-NMR spectra of GL-A produced by Sporisorium sp. aff. sorghi SAM20 (a) and MEL-A produced by Moesziomyces antarcticus T-34 (b). The spectra were recorded at 400 MHz using CDCl3 as solvent. The MEL-A spectrum was adopted from previously reported data by Fukuoka et al. 2008a.
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Figure S3 1H-NMR spectrum of GL-A produced by Sporisorium sp. aff. sorghi SAM20. The spectrum was recorded at 400 MHz using CDCl3 as solvent.
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Figure S4 1H-NMR spectrum of GL-A produced by Sporisorium sp. aff. sorghi SAM20. The spectrum was recorded at 400 MHz using CDCl3 as solvent.
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Figure S5 13C-NMR spectrum of GL-A produced by Sporisorium sp. aff. sorghi SAM20. The spectrum was recorded at 100 MHz using CDCl3 as solvent.
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Figure S6 DEPT 135 and 13C-NMR spectra of GL-A produced by Sporisorium sp. aff. sorghi SAM20. The spectra were recorded at 100 MHz using CDCl3 as solvent.
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Figure S7 1H–1H COSY spectrum of GL-A produced by Sporisorium sp. aff. sorghi SAM20. The spectrum was recorded at 400 MHz using CDCl3 as solvent.
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Figure S8 HSQC spectrum of GL-A produced by Sporisorium sp. aff. sorghi SAM20. The spectrum was recorded at 400 MHz using CDCl3 as solvent.
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Figure S9 HMBC spectrum of GL-A produced by Sporisorium sp. aff. sorghi SAM20. The spectrum was recorded at 400 MHz using CDCl3 as solvent.
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Figure S10 LC–ESI–MS chromatogram of GL-A produced by Sporisorium sp. aff. sorghi SAM20
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Figure S11 GC–MS total ion current of GL-A produced by Sporisorium sp. aff. sorghi SAM20
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