Supporting Table 1. Performance summary of qHTS

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Supporting Table 1. Performance summary of qHTS

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Supporting Table 1. Performance summary of qHTS assays. qHTS Assay. Cell Line. Z'. Intraplate Control. AC. 50. [ÂµM] grp78-luciferase primary. U87-MG. 0.41 Â± ...

Supporting Table 1. Performance summary of qHTS assays. qHTS Assay

Cell Line

Z'

Intraplate Control

AC50 [µM]

U87-MG

0.41 ± 0.21

Thapsigargin

0.051 ± 0.042

Thapsigargin

0.066 ± 0.13

U87-MG

0.36 ± 0.19 Tunicamycin

0.014 ± 0.006

Thapsigargin

NA

Tunicamycin

0.41 ± 0.007

17-AAG

7.75 ± 0.38

Thapsigargin

0.051 ± 0.005

Thapsigargin

0.020 ± 0.022

Tunicamycin

0.016 ± 0.016

Thapsigargin

0.002

Tunicamycin

0.004 ± 0.0017

Thapsigargin

0.00025 ± 0.000173

Tunicamycin

0.0041 ± 0.001

Thapsigargin

< 10

Tunicamycin

< 1.6

Thapsigargin

< 10

Tunicamycin

< 1.6

Thapsigargin

< 10

Tunicamycin

< 1.6

grp78-luciferase primary grp78-luciferase secondary

UPRE-luciferase

Caspase 3/7

Cell viability

TSCER2

U87-MG

0.78 ± 0.21

U87-MG

0.48 ± 0.19

JHH-136

JHH-520

U87-MG

3D Cell viability

JHH-136

JHH-520 Calcium mobilization

0.68 ± 0.06

0.43 ± 0.15

0.51 ± 0.08

0.66 ± 0.09

0.32 ± 0.03

0.61 ± 0.12

U87-MG

0.70 ± 0.15

A23187

NA

JHH-136

0.64 ± 0.13

Thapsigargin

0.03 ± 0.02

JHH-520

0.79 ± 0.05

Thapsigargin

0.0041 ± 0.004

Matrix