Synthesis and characterization of iron oxide-chitosan magnetic nanoparticles and application for MR imagine biomedical contrast agents Robert Lian-Huey Liua, g, Rui-Qi Leeb, Keng-Liang Ouc,e,f, Chi-Ming Leed, Wen-Tien Hsiaod, Yu-Jing Dingd, Jia-Yu Yangd, Ben-Je Lwob, Ming-Der Gera点 a
Graduate School of Defense Science, Chung-Cheng Institute of Technology, National Defense University, Tao-Yuan, 335, Taiwan, R.O.C. b
Department of Mechatronic, Energy, and Aerospace Engineering, Chung-Cheng Institute of Technology, National Defense University, Tao-Yuan, 335, Taiwan, R.O.C. c d
Research Center for Biomedical Devices, Taipei Medical University, Taipei 110, Taiwan, R.O.C. Department of diagnostic radiology, Taipei Medical University Hospital, 252, Wu-Hsing Street, Taipei, 110, Taiwan, R.O.C. e
Graduated Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei 110, Taiwan, R.O.C.
f
Research Center for Biomedical Implants and Microsurgery Devices, Taipei Medical University, Taipei 110, Taiwan, R.O.C.
g
Department of Chemical and Materials Engineering, Minghsin University of Science and Technology, Hsin-Chu, 304, Taiwan, R.O.C. *
Corresponding Author¶s E-mail:
[email protected]
Keywords烉 Magnetic resonance imaging; Biomedical contrast agent; In situ Co-precipitation; Iron oxide-chitosan; In vitro/In vivo; Magnetic nanoparticles
Extended Abstract The magnetic nanoparticles such as iron oxide which is superparamagnetic, and biocompatible colloid have attracted for the application in the field of biomedical and diagnostic. It is important in biomedical application for the nanoparticles with narrow size distribution and good dispersion. For the protecting and stabilization of iron oxide nanoparticles, chitosan and/or some other polymers have been used as coating agent to achieve excellent dispersion. Concedering the circulation of nanoparticles in the body as shorter as better, the polymer coated nanoparticles with the diameter preferable small than 30nm. The synthesis of magnetic iron oxide/iron oxide-chitosan nanoparticles by the method of co-precipitation/ in situ co-precipitation which were prepared by the ferric sulfate and ferrous sulfate as the main salt, citric acid as the surfactant, and in situ coating with chitosan.
The effect of different concentrations of surfactants for the size of
magnetic nanoparticles was investigated. The results of X-ray powder diffraction (XRD) to identify the iron oxide magnetic nanoparticles crystal orientation and particle size, transmission electron microscopy (TEM) to observe the average size about 10焍20 nm, superconducting quantum interference device (SQUID) to identify the saturation magnetization and coercivity of magnetic particles, which saturation magnetization measured up to 52 emu / g . The effect of the signal intensity in MR image with the concentration of iron oxide-chitosan was observed by the in vitro experiment. the several concentration of iron oxide-chitosan, 1%, 0.5%, 0.25%, 0.125%, 0.058%, 0.029%, by diluted with DI water and keep the neutral pH. Fig.1 showed the signal intensity decrease with lower concentration. The in vivo application of iron oxide-chitosan were conducted in accordance with guidelines for the use and care of Proc. of the 14th Asia Pacific Confederation of Chemical Engineering Congress Editors: Chi-Hwa Wang, Xin Wang and Praveen Linga. Copyright © 2012 APCChE Organisers. All rights reserved. Published by: Research Publishing ISBN: 978-981-07-1445-1 doi:10.3850/978-981-07-1445-1 766 68
Proc. of the 14th Asia Pacific Confederation of Chemical Engineering Congress
laboratory animals. To investigate the images of magnetic resonance before and after the iron oxide-chitosan which was given as an intravenous injection to the mouse liver. As shown in the Fig. 2(C) and (D), comparing the T2 and T2-weighted MR images acquired before and after injection, there are a remarkable decrease in signals intensity in liver which can be explained that after injection, iron oxide-chitosan nanoparticles are detected and phagocytosed by macrophages. It would be suggested that the iron oxide-chitosan prepared by the current synthetic method have good biocompatibility and can be potential used as T2 type contrast agents for the MRI application.
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Fig 1. In vitro MRI phantom images with different concentration of iron oxide-chitosan nanoparticles.
Fig 2. In vivo images before and after the injection of iron oxide-chitosan nanoparticles to the mouse liver. 69
