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Comment

mouse pri-miR-141. 185 bp mouse pri-miR-200a. 201 mouse pri-miR-429. 170 bp mouse pri-miR-2000. 284 bp. Mouse Zeb2. 223 bp mouse pri-miR-200b.

Table S3. Primers used for Realtime PCR and RT-PCR Gene name Mouse Epcam

Primers GAGTCCCTGTTCCATTCTT

Product length 250 bp

TCTCCTTTATCTCAGCCTTC

Mouse E-Cadherin

GTCTCCTCATGGCTTTGC

228 bp

CTTTAGATGCCGCTTCAC

Mouse Twist1

AGCGGGTCATGGCTAACG

162 bp

GGACCTGGTACAGGAAGTCGA

mouse pri-miR-141

GGTCAGTTCCTTCAGCAGTG

185 bp

CCAAATGTGTTTCCAAATCT

mouse pri-miR-200a

CCGAAGACAGGCTGATGATA

201

GTGACAATCCACTTCTACTC

mouse pri-miR-429

ACTGCCCTGCTGATGGATGT

170 bp

ACAGTTGTAACAGATGGATT

mouse pri-miR-200c

TGATCTTGAAGGTGGACTGG

284 bp

CACTGGATTGGAGGAGGG

Mouse Zeb2

GAGCTTGACCACCGACTC

223 bp

TTGCAGGACTGCCTTGAT

mouse pri-miR-200b

tgcagggctttctgctgttgtcc cacccatatcgccaaggctaact

Mouse Foxc2

TCGACCCGGACTCCTACAA

255 bp

GGCTCAGCGTCTCCACCTT

mouse beta-actin

CTGTCCCTGTATGCCTCTG

218 bp

ATGTCACGCACGATTTCC

Mouse DSG2

ACGGGCAGATAACAGACA

273 bp

GGGTCACAATCCCTTCAT

Mouse DSP

GCAACAAGCCCATTATCC

203 bp

GCAAGAAAGGTCCACAGC

Mouse Pkp2

GCAGCCGAAGTAGGAAAC

216 bp

CCACCAATGTGGGTATCG

Mouse Cldn3

GGCGGCTCTGCTCACCTTA

116 bp

CGTACAACCCAGCTCCCATC

Mouse cldn4

GGGAATCTCCTTGGCAGTC

190

GGCGAGCATCGAGTCGTA

Mouse Grhl2

TTTGGTCCAACACCGTCTA

162 bp

CACTGGCAGCCCATACTT

Mouse GAPDH

GTTGTCTCCTGCGACTTCA

184 bp

GGTGGTCCAGGGTTTCTTA

Mouse cldn7

GGGAGATGACAAAGCGAAGA CAGAAGGACCAGAGCAGACC

208 bp

Mouse Gjb3

AGCATCGCCAGAAGCACG

278 bp

GGCAGAAGCGCCTACCAT

Mouse ocln

ATCCACCTATCACTTCAGA

292 bp

TAATCTCCCACCATCCTC

Mouse Tjp2

AAGTTCCCTGCCTACGAG

189 bp

ATTCAACCGAACCACTCC

Mouse Wnt7A

GGCAACCTGAGCGACTGT

457 bp or 302 bp

AGTAATTGGGTGACTTCTCG

Mouse Snai1

GTCGTCCTTCTCGTCCACC

391 bp

GGCCTGGCACTGGTATCTC

Mouse Snai2

TCCCATTAGTGACGAAGA

204 bp

CCCAGGCTCACATATTCC

Mouse Axin2

AGCCTAAAGGTCTTATGTGG

287 bp

ATGGAATCGTCGGTCAGT

Mouse Esrp1

TATTGAATCACCAGGGACG

269 bp or 257 bp

TTCGGTAGGAATCACAGC

Mouse CD44

GTACAGAAGGTGGTTGTGAT

To detect alternative splicing 612 bp, 494 bp, 205 bp

AGGTGGAGGGAATACTGG

333 bp, 396 bp

GAAGGGCGAGTATAGAAC AGATGCCAAGATGATGAG

Mouse CTNND1 Mouse Enah

GAACGAGGAAGAGGAAGA

GCACTTGGCTGTGATGAA

Mouse MAP3K7

CTGCCACAAACGACACTA

328 bp, 247 bp

GTCCCAGTAACAGTCAAGTC

Mouse ID2

GGTGGACGACCCGATGAGT

268 bp

TGCCTGCAAGGACAGGATG

Mouse Zeb1

CCATACGAATGCCCGAACT

386 bp

ACAACGGCTTGCACCACA

Mouse TCF4

CACAACGGAGCGATGGGTA

195 bp

GGGTGGGTTCAAGTCAGG

Human Wnt7A

AGGGCAACCTGAGCGACTG

585 bp

GGGCGTACTGGTGGGTGTT

Human beta Actin

CACCAACTGGGACGACAT

189 bp

ACAGCCTGGATAGCAACG

Human GAPDH

GGATTTGGTCGTATTGGG

205 bp

GGAAGATGGTGATGGGATT

Human E-Cadherin

CTGAGAACGAGGCTAACG

289 bp

TTCACATCCAGCACATCC

Human CD24

TACCCACGCAGATTTATT

162 bp

AGAGTGAGACCACGAAGA

Human Epcam

ATCGTCAATGCCAGTGTA

288 bp

CTCGCTCAGAGCAGGTTA

Human Grhl2

CTTGACATTGCCGATTAC

224 bp

ATGGGTTTATTGCTACGATT

Human pri-miR-200a

CTTACCGGACAGTGCTGGAT

72 bp

GCGGGTCACCTTTGAACATC

Human pri-miR-200b

AGCTTCCCAGCGAGTCCCA

133 bp

TCCGCCGTCATCATTACCAG

Human pri-miR-200c

GTGTTTGGGTGCGGTTGG

83 bp

AGGCGATGGATGTTGCTGAC

Human pri-miR-141

CCACCCAGTGCGATTTGT

234 bp

CGGGAGCCATCTTTACCAG

Human vimentin

CCAGGCAAAGCAGGAGTC

213 bp

CGAAGGTGACGAGCCATT

Human Zeb1

GTGGCGGTAGATGGTAAT

254 bp

CTGTTTGTAGCGACTGGA

Human Zeb2

ACCAGCCCTTTAGGAGTT

272 bp

AGACCGACAGGCGGAATA

Human Sani1

CCCCAATCGGAAGCCTAA

261 bp

CCTTTCCCACTGTCCTCAT

Human Snai2

TCCTGGTCAAGAAGCATT

274 bp

GAGGAGGTGTCAGATGGA

Human Twist1

CGGGAGTCCGCAGTCTTA GCTTGAGGGTCTGAATCTTG

161 bp