Table S6 Reaction Nr. Reaction kon (M-1 s-1) koff - PLOS

Table S6 Reaction Nr. 1 2 3 4 5 6 8 9 10 11

Reaction A + GEF ↔ A_GEF A_GEF → GEF_act GEF_act + RasD ↔ GEF_act_RasD GEF_act_RasD → GEF_act + RasT RasT + Raf ↔ RasT_Raf RasT_Raf → RasT + Raf_act Raf_act → X RasT + GAP ↔ RasT_GAP RasT_GAP → RasD + GAP X_act → X_deg

kon (M-1 s-1) 10e6

koff (s-1) 0.6

15e6

1.3

29.6e6

5.7

k forw (s-1) 1 0.1434 0.7624 1

2.854e6

0.96 7.76 0.001

remarks estimation estimation Schoeberl, 2002 Yamada, 2004 Kiel, 2003 Yamada, 2004 estimation Yamada, 2004 Yamada, 2004 estimation

Kiel C (2003) Investigation of Ras-effector complexes with changed electrostatic properties. PhD thesis, Ruhr-Universitaet Bochum, Germany. Schoeberl B, Eichler-Jonsson C, Gilles ED, Mueller G (2002) Computational modeling of the dynamics of the MAP kinase cascade activated by surface and internalized EGF receptors. Nat Biotech 20: 370-375. Yamada S, Taketomi T, Yoshimura A (2004) Model analysis of difference between EGF pathway and FGF pathway. Biochem Biophys Res Commun 314: 1113-1120.