extracted nuclei from snap frozen buffy coat stored for the purpose of molecular ... by lysing erythrocytes with lysing solution (LYSE S III DIFF, Coulter Corp.).
Available online at www.annclinlabsci.org
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Annals of Clinical & Laboratory Science, vol. 37, no. 1, 2007
Technical Note : Fluorescence In Situ Hybridization (FISH) Using Snap Frozen Buff y Coat
Hun Jung Min,1,4,5 Jinkyoung Lee,2 Jung Eun Choi,1 Su Shin,3 and Dong Soon Lee1,4,5 1 Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea 2 Department of Laboratory Medicine, Korea Cancer Center Hospital Seoul, Korea 3 Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea 4 Cancer Reseach Institute, Seoul National University College of Medicine, Seoul, Korea 5 National Research Laboratory for Molecular Cell Imaging, Seoul, Korea Abstract. We report the successful application of interphase fluorescent in situ hybridization (FISH) using extracted nuclei from snap frozen buff y coat stored for the purpose of molecular analysis. Included in this study were 30 frozen buff y coat specimens, ranging from
