Testing Conditions for Determination of Minimum Fungicidal ...
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Testing Conditions for Determination of Minimum Fungicidal ...
Mar 7, 2002 - Denning, D. W., K. Venkateswarlu, K. L. Oakley, M. J. Anderson, N. J. ... Espinel-Ingroff, A., M. Bartlett, V. Chaturvedi, M. A. Ghannoum, K. C..
Testing Conditions for Determination of Minimum Fungicidal Concentrations of New and Established Antifungal Agents for Aspergillus spp.: NCCLS Collaborative Study A. Espinel-Ingroff,1* A. Fothergill,2 J. Peter,3 M. G. Rinaldi,2 and T. J. Walsh3 Medical College of Virginia Campus of Virginia Commonwealth University, Richmond, Virginia1; University of Texas Health Science Center, San Antonio, Texas2; and National Cancer Institute, Bethesda, Maryland3 Received 7 March 2002/Returned for modification 16 April 2002/Accepted 13 June 2002
Standard conditions are not available for evaluating the minimum fungicidal concentrations (MFCs) of antifungal agents. This multicenter collaborative study investigated the reproducibility in three laboratories of itraconazole, posaconazole, ravuconazole, voriconazole, and amphotericin B MFCs for 15 selected isolates of Aspergillus spp. After MIC determinations for the 15 isolates in each center by the NCCLS M38-A broth microdilution method with four media, standard RPMI 1640 (RPMI), RPMI with 2% dextrose, antibiotic medium 3 (M3), and M3 with 2% dextrose, MFCs were determined for each isolate-medium-drug combination. MFCs were defined as the lowest drug dilutions that yielded
