CMML-0 CMML-1 CMML-2 lien LMMC1 ou2. CMML-0. CMML-1. CMML-2. 0.01. 0.1. 1. 10. 100. miR-150 expression (relative to RNU6B) h sa. -miR. -1. 5. 0 e xp.
Supplementary informations A miR-‐150 / TET3 pathway regulates the generation of mouse and human non-‐ classical monocyte subset Selimoglu-‐Buet D. et al.
Selimoglu-Buet D et al, Supplementary Figure 1 (related to Figure 1) B
miR expression miR expression (relative to HPRT) (relative to PPIA)
hsa-miR-150 miR expression miR expression (relative to PPIA) (relative to RPL32)
● ●
●
MUT_AML
CTL CMML CTL
10
7.5
log2(I)
7.0 6.5
●
5.5
5.0
CTRL
●
log2(I)
7.5 7.0
log2(I)
●
5.0
5.0
0
11.0 10.0 9.0 8.0
●
●
6.0
6.0 5.5
5
7.0
7.0 6.5
10
hsa−miR−451
8.0
6.0
Log2(I)
15
C
hsa-miR-494
hsa-miR-451
●
6.0
A
WBC 9 (10 /L) (10 WBC /L)
60 40
1
10
1 1000.01
100
0.1
1
10
100
10
100 10 1 0.1 0.01 CMML-0 CMML-0
9
10
1
10
100
15 10 5 0 0.01
G 0.1
1
10
100
100
10
0.1
1
10
100
0.01
0.1
1
hsa-miR-150 expression (/RUN6B)
10
100
CMML-1
CMML-1
CMML-2
CMML-2
1000 100
TET2
SRSF2
ASXL1
RAS
RUNX1
10 1 0.1 0.01
WT MT WT MT WT MT WT MT WT MT
2 Te wt t2 SR m SF ut SR 2 W SF T AS 2 m XL ut AS -1 XL WT -1 m RA ut S RA WT S AM m L- ut AM 1 W L- T 1 m ut
0.1
0.1 10000.01
Platelets (10 /L)
0.1 200.01
1
Te t
1
9 9/L) Platelets (10
Hemoglobin (g/dL) Hb (g/dL)
10
Neutrophils 9/L) (10 PN (10 /L)
0.1
9
Monocytes 9/L) (10 Monocytes count (10 /L)
20 0 1000.01
100
9
Age (year) age (year)
80
lien LMMC1 ou2
miR-150hsa-miR-150 expression (relative to RNU6B) expression (/RUN6B)
F
1000
100
hsa-miR-150 expression (/RUN6B)
E
Supplementary Figure 1. Differen4al miRNA expression profiles in healthy controls or CMML sorted peripheral blood monocytes and rela4onship with clinical and biological features. A. Hierarchical clustering of miRNA profiles obtained by microarray analysis (learning cohort), established using the Euclidean distance method and the Ward clustering method, and validated by boostraping (package pvclust). B. Box plot showing the variaEon in hsa-‐miR-‐150, hsa-‐miR-‐451 and hsa-‐miR-‐494 expression in samples of the learning cohort analyzed in figure 1A (centre line : median, whiskers: min to max). C. Box plot showing the relaEve expression of hsa-‐miR-‐150, hsa-‐miR-‐451 and hsa-‐miR-‐494 as measured by qRT-‐PCR in CD14+ cell samples used for microarray analysis (learning cohort). Normalized to L32 or PPIA (CTL=5; CMML=33), centre line : median, whiskers: min to max. D. qRT-‐PCR analysis of hsa-‐miR-‐150 and hsa-‐ miR-‐451 expression in healthy donor and CMML paEent CD14+ cells of controls and CMML paEents (validaEon cohort). hsa-‐miR-‐150 normalized to PPIA (CTL=19; CMML=124) or HPRT (CTL=19; CMML=143). hsa-‐miR-‐451 normalized to PPIA (CTL=24; CMML=44) or HPRT (CTL=24; CMML=52). Red lines: median with interquarEle range. Mann-‐Whitney test: * P
