May 7, 2017 - 5/7/2017 Abstract: The Effects of Different Bioprocessing Parametrs on ... pH cultivation condition of 5.5 under two different feeding solutions.
5/7/2017 Abstract: The Effects of Different Bioprocessing Parametrs on Pectinase Production By Aspergillus Niger from Orange Peel Waste (Rapid Fire) (201…
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427104 The Effects of Different Bioprocessing Parametrs on Pectinase Production By Aspergillus Niger from Orange Peel Waste (Rapid Fire)
AtAGlance Wednesday, November 11, 2015 Browse by Day Browse by Topics Author Index Keyword Index Personal Scheduler
Exhibit Hall 1 (Salt Palace Convention Center) Nor Zalina Othman 1, Hamiza Suhaimi2, Roslinda Abd Malek1, Solleh Ramli3, Ramlan Aziz4, Mona A Esawy5 and Hesham EL Enshasy1, (1)Institute of Bioproduct Development, Universiti Teknologi Malaysia, Johor Bahru, Malaysia, (2)Bioprocessing Enginering Laboratory, Universiti Teknologi Malaysia, Johor Bahru, Malaysia, (3)Institut of Bioproduct Development, Universiti Teknologi Malaysia, Johor Bahru, Malaysia, (4)Institute Bioproduct Development, Universiti Teknologi Malaysia, Skudai, Malaysia, (5)Department of Chemistry of Natural and Microbial Products,, National Research Center, Dokki, Egypt Pectinase is a heterogeneous group of enzymes that have a polysaccharides substrate and breaks down the pectin component which found in the plant cell wall into simple sugar and galacturonic acid. The pectinases or usually known as pectinolytic enzymes were extensively used in food industries that involve degradation of plant materials to fasten the fruit juice extraction process. Among different biofactories of pectinases, filamentous fungi such as Aspergillus niger were best known for the production and secretion of pectinase. Therefore, the objective of this research was to develop industrial culture medium and a cultivation strategy for the production and secretion of pectinases in a semiindustrial scale 16L bioreactor by A. niger. In this study, the effect of medium composition on the production and secretion of pectinase was studied through the onefactoratatime (OFAT) method and statistical approach. Therefore, the pectinase production from the new optimized production medium when pectin from orange peel as the carbon source which produced more than 64.83 % of pectinase when compared to unoptimized medium. Followed by batch cultivation in the semiindustrial scale 16L bioreactor under uncontrolled pH yielded 51.35 % more pectinase compared with the cultivation under controlled pH of 5.5. Finally, the fedbatch cultivations employing a constant feeding mode under uncontrolled pH with the initial pH cultivation condition of 5.5 under two different feeding solutions which were full medium compositions of the production medium and concentrated sucrose. The higher pectinase yield was produced when feeding with concentrated sucrose about 429.95 U mL1 of pectinase activity compared to the full medium feeding only 138.27 U mL1. These all together will lead to the development of industrial process for pectinase production in semi industrial scale bioreactor. Therefore, A. niger is best cultivated in pHuncontrolled condition with DO consistent at 30 % level using the constant feeding strategy of concentrated sucrose. Extended Abstract: File Not Uploaded See more of this Session: Poster Session: Food and Bioprocess Engineering See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division
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