Jan 30, 2017 - dimension obtained in PhotoShop was converted to mm2 area of the retina and expressed ..... Interestingly, VEGF levels are upregulated during the time course of retinal .... fluid in advanced stage retinopathy of prematurity on.
The Vasoproliferative Phase in the Oxygen-Induced Rat Model of Retinopathy of Prematurity Exhibits Concomitant Increased Expression of VEGF164 and Phosphorylation of eNOS at Serine1179. Jorge L Jacot1,2*, Robin C Looft-Wilson3, Nazita Yousefieh4, Frank A Lattanzio5, Alireza Hosseini5 Department of Pathology and Anatomy, Eastern Virginia Medical School, USA Angioceutics International LLC, USA 3 Department of Kinesiology and Health Sciences, College of William and Mary, USA 4 Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynaecology, USA 5 Department of Physiological Sciences, T.R. Lee Center for Ocular Pharmacology, Eastern Virginia Medical School, USA 1 2
Abstract Purpose: The nitric oxide pathway requires phosphorylation of eNOS to liberate NO. We correlated mRNA/protein levels of VEGF164, and phosphorylated Serine1179 of eNOS during ROP vasoproliferation. Methods: Animals were exposed to oxygen (50/10% every 24-hours) for 14 post-natal (PN) days. Pups were continuously exposed to 20.9% ambient oxygen from PN15 and enucleated on PN days 15, 18, and 20. ADPase stained retinas were analyzed for degree of avascularity/total retina area. Western-blotting quantified the phosphorylation of eNOS at Ser1179, the ratio of total eNOS to ERK2. Immunoreactivity of VEGF and of eNOS serine1177 determined tissue protein expression. Results: Percent retinal avascularity was reduced (p
