THIS VERSION IS FOR THE 4th EDITION OF GARRETT AND ...

THIS VERSION IS FOR THE 4th EDITION OF GARRETT AND GRISHAM’S BIOCHEMISTRY Lecture outline with recommended reading: (Note: “BC” indicates a background concept; “AC” indicates an advanced concept; and, “NIT” indicates not covered in text.)

Outline I

Background Material—chemistry of water

II

Biological Macromolecules A. Protein Structure 1. Amino Acids i. Amino acid structure ii. BC: Acid/Base chemistry 2. Primary Structure i. Nomenclature/Chemistry ii. Determining primary structure iii. pH in polypeptides (pI) 3. Secondary Structure 4. Tertiary Structure 5. Quaternary Structure 6. Determining protein tertiary structure B.

C.

D.

Protein Function 1. Overview 2. Enzymes i. BC: Thermodynamics ii. Enzyme Basics iii. Kinetics iv. Inhibition v. Generalized enzyme kinetic mechanisms vi. Active site architecture & chemistry

Carbohydrates 1. BC: Stereochemistry 2. Monosaccharides 3. Oligosaccharides 4. Glycoproteins/Proteoglycans Lipids 1. 2.

3. E.

F.

Types/Structures Biological Membranes i. Structure ii. Transport Lipidomics

Nucleic Acid Structure 1. Primary structure 2. Secondary structure 3. Tertiary Structure 4. RNA

Section(s)

Pages

§2.1

28-35

§4.1 §4.2

70-76 76-82

§5.1 §5.4-5.5 NIT §6.1-6.3 §6.4 §6.5 & §5.7 NIT

93-97 99-117

§5.8

120-123

§3.1-3.8 §13.1 §13.2-13.3 §13.4 §13.5 §13.6, §14.1-14.6 + NIT

48-68 382-386 386-397 397-403 403-409 409-410, 419-447

§7.1-7.2 §7.3-7.4 §7.5-7.6

181-191 191-204 204-213

§8.1-8.8

219-236

§9.1-9.2 §9.3-9.10 §8.9 + NIT

242-260 260-287 237-239

§10.1-10.6, 11.1 §11.2-11.3 §11.4-11.5 §11.7

291-313, 316-320 320-333 333-339 341-350

Nucleic Acid Function (General Overview Only—details covered in 443/543) 1. DNA Replication §28.1-28.3 2. AC: Teleomeres §28.5, Box p. 340 3. DNA Transcription §29.1 4. Operons §29.2 (portion)

134-146 146-173 173-178, 119-120

862-871 874-876 (340) 906-912 912-924

5. 6. 7. 8. III

RNA processing in eukaryotes Translation: Protein synthesis RNA Function AC: Recombinant DNA AC: RNAi

§29.5 §30.1-30.6 §10.5 (portion) §12.1-12.4 §12.5-12.6

939-946 952-980 303-307 354-375 375-379

Metabolic Processes A.

Overview

§17.1-17.4

511-529

B.

Major processes of Glucose Metabolism 1. Glycolysis

§18.1-18.8

535-560