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Toward an optimal sampling protocol for Hemiptera on understorey ...

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lected from the tray with forceps or a paintbrush dipped in ethanol. ... Sticky trapping has been used to monitor pop- ulations of ...... global network. J. Insect ...
Journal of Insect Conservation (2005) 9:3–20 DOI 10.1007/s10841-004-2351-y

 Springer 2005

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Toward an optimal sampling protocol for Hemiptera on understorey plants Melinda L. Moir1,*, Karl E.C. Brennan1, Jonathan D. Majer1, Murray J. Fletcher2 and John M. Koch3 1

Department of Environmental Biology, Curtin University of Technology, G.P.O Box U1987, Perth, WA 6845, Australia; 2Orange Agricultural Institute, Forest Road, Orange, NSW 2800, Australia; 3Alcoa World Alumina Australia, P.O. Box 252, Applecross, WA 6953, Australia; *Author for correspondence (e-mail: [email protected]; phone: +61-8-9398-1286; fax: +61-8-9266-2495) Received 24 February 2004; accepted in revised form 22 August 2004

Key words: Chemical knockdown, Inventorying biodiversity, Sampling methods, Vacuum sampling

Abstract There are no standardised sampling protocols for inventorying Hemiptera from understorey or canopy plants. This paper proposes an optimal protocol for the understorey, after evaluating the efficiency of seven methods to maximise the richness of Hemiptera collected from plants with minimal field and laboratory time. The methods evaluated were beating, chemical knockdown, sweeping, branch clipping, hand collecting, vacuum sampling and sticky trapping. These techniques were tested at two spatial scales: 1 ha sites and individual plants. In addition, because efficiency may differ with vegetation structure, sampling of sites was conducted in three disparate understorey habitats, and sampling of individual plants was conducted across 33 plant species. No single method sampled the majority of hemipteran species in the understorey. Chemical knockdown, vacuum sampling and beating yielded speciose samples (61, 61 and 30 species, respectively, representing 53, 53 and 26% of total species collected). The four remaining methods provided species-poor samples (

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